National Flow Cytometry Resource (NFCR), Bioscience Division This collaborative position between Los Alamos National Laboratory and the University of New Mexico Pathology Department led to the development and characterization of multiplex microsphere based flow cytometry assays to detect activity and inhibition of pathogenic proteases. High-throughput screening efforts of protease assays developed in this position led to the identification of inhibitors of Botulinum Neurotoxin Type A. These assays have also been adapted to other proteases of interest for pharmacological targeting. Working under Dr.Steve Graves as a postbachelor researcher this position involved developing yeast display techniques to monitor bacterial toxin protease activity through flow cytometry. This position led directly to a graduate student researcher position at LANL continuing this project in a multiplex microsphere format. the laboratory of Dr. Tom H. Stevens I conducted research on the mechanisms of membrane protein transport in Saccharomyces cerevisiae particularly the role and function of the model organism's open reading frame Yol 129 in budding yeast. In this position I worked under mycologist Dr. George Carroll to develop a PCR based test to diagnose infection of citrus orange crops with a pathogenic fungus known as citrus blackspot.

Highlights:

• Research
• Biomedical Industry
• Construct (App Dev Tool)
• Diagnostics
• Product Development
• Molecular Bilogy
• Flow Cytometry
• Protein Engineering