Description
Profile Cell and Molecular Biologist with the passion and expertise essential for success in Research. Industry experience developing and validating pre-clinical differentiated stem cells for phI/II clinical trial in AMD and Startsgart Macular Degeneration treating the leading cause of blindness. Industry experience performing pre-clinical research for a global pharmaceutical company. I can offer a significant contribution in medical research, screening and development as a Senior Research Scientist. Adept at novel assay development, most especially cell-based assay, for target disease model and discovery therapeutics. Adept at Molecular Biology, Biochemistry Techniques, Cell Assay (Proliferation, Biomarker..), Cell Biology, Signal Transduction, Cell culture. Tasked with developing cell based assays, both traditional and radiometric, to understand the mechanism of action as well as pathways specific to renal fibrosis to further elucidate current anti-fibrotic compounds. Successfully contributed to obtaining FDA clearance to proceed with two separate Phase 1/2 clinical trials to test the safety of the hESC-derived RPE cellular therapy for Stargardt's Macular Dystrophy (SMD) and for Dry Age-Related Macular Degeneration (Dry AMD) Cellular Biology / Cell Culture Human PBMC isolation, Human Embryonic Stem Cell Biology, Transfection RNA Interference Assay Luciferase Assays.. Immunofluorescence..Proliferation Assay Cell Biology primary cell line, tissue outgrowth cell development and immortalized cells Cell based assays, high throughput screening, invasion assays, immunofluorescence, immunohistochemistry, etc Molecular Biology / Biochemistry QC Technical Writer and Document Specialist Molecular Biology RNA/DNA isolation from cells and tissue, rt-PCR, primer design, PCR optimization, Northern Blot analysis, gel electrophoresis Protein Biochemistry, lysate, Western Analysis, ELISA and Meso Scale Discovery platform, SpectraMax and SoftMaxPro Flow Cytometery RNA (Northern blot) DNA (Cloning, RT-PCR, PCR, Southern blot) Protein (western blot, immunoprecipitation, ELISA, kinase assay, protein expression, and purification). Independently authored as a Product Development Scientist/QC Technical Writer over 100 QCS/QCF documents used to characterize and qualify products and processing and executing assay development. ( SOP, protocols and publication manuscripts) Edited, reviewd and co authored pre - IND and IND filings to the FDA working with Clinical Trials group Matrix management skills, management and development of information systems for handling data from multiple departments Proficient in Word, Excel, PowerPoint, Photoshop, Softmax Pro, MSD Discovery Workbench and standard scientific software, Sequence Explorer, Expression Explorer, etc
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