Description
Profile Research scientist with 15 years of post-PhD experience in molecular biology, cell biology, biochemical, and imaging techniques to screen new molecules and drugs for potential in treating various cancers and neurological diseases. First and co-author of "https://scholar.google.com/citations*hl=en&user=LoFuX7UAAAAJ&view_op=list_works&sortby=pubdate" 44 publications with over 1852 citations. Secured research funding for several principal Investigators. Extensive experience working collaboratively and across disciplines, frequently assisting industry professionals in testing new products for quality control and preparation for manufacturing. Has over eight years' experience training and supervising undergraduate, graduate, and medical students in research techniques. Areas of Expertise ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Gene and recombinant DNA cloning, DNA, RNA and protein extraction in cells and tissues * Genomic PCR, ISH, RT-PCR and quantitative PCR * Primary cell cultures, three-dimensional (3D) explants, stem cells and dissociated neurons * Cell culture of several primary cancer cell lines (breast, prostate, skin), as well as hippocampal stem cells, and live cell imaging by using confocal, bright field and fluorescence microscopy and time lapse video compilations. * Double and triple immunofluorescence of cells and tissue sections. * Fluorescence microplate readers, luminometers and cell lysis techniques. * DNA, RNA transfection, RNAi (with siRNA and shRNA), flow cytometry * Western blot analysis, ELISA, in vivo and in vitro labeling of proteins with radioactive isotopes and high throughput (HTS) screening assays * Mouse and rat dissections, Geno-typing, mouse models. * Undergone lab safety, biohazard, radioactive and animal training. * Research and Analysis * Write animal protocols. * Cost Reduction Process * Sample Collection * Staff Training * Safety Standards * Records Accuracy * Proofreading/Editing * Technical Manual Compilation * Policy Implementation * Time Management * Effective Multitasking * Microsoft Word, Excel, PowerPoint, and Endnote * Adobe Photoshop, Adobe Illustrator. * Image J, Metamorph, Softmax and Graph Pad. Familiar with imaris. * Writing animal protocols for IACUC approval.
Work Experience
COMPANY | POSITION HELD | DATES WORKED |
---|---|---|
(Confidential) | Senior Research Associate | 2/2005 - Present |
Beckman Research Institute | Post Doctoral Fellow | 9/2003 - 11/2004 |