Description
Summary: Experienced cell/molecular biologist with background in bioanalytics, cell-based & ligand bind- ing assay development/validation, early discovery of therapeutic biologics and anticancer drugs. Expert in Mesoscale Discovery MSD multiplex/singleplex platform. Highly motivated, analytical, creative worker. Effective independent researcher and a team contributor. Research Expertise Biomarker analysis, PK & PD experience * Strong experience with preparation & homogenization animal tissue samples (brain, GI tract, skin, cornea, vitreous/retina, whole eye). * Extensive experience in developing cell-based and in vitro ligand/antibody-binding as- says to measure the levels of proteins/analytes in pre-clinical samples from pharma- cokinetic (PK) and pharmacodynamics (PD) studies. * Extensive experience in human/animal tissue sample biomarker profiling using multi- plex/singleplex MSD technology and ELISA assays. Cell Biology * Strong experience in antibody and bi-specific antibody engineering and drug discovery * Expert in cell biology and culturing a variety of cell lines. * Expert in generation of transient and stable cell lines expressing recombinant proteins and IgGs. * Expert in analysis of cell cycle, cell death, proliferation, cytotoxicity and apoptosis as- says (caspases, cleaved PARP, Annexin-V and DNA fragmentation), MTT assay, IC50 determination. * Expert in mammalian cell transfection, siRNA and shRNA technology, cDNA overex- pression including transient, constitutive and inducible systems. * Experience in using retroviral and lentiviral vectors. * Experienced in visual and fluorescence microscopy. Molecular Biology and Biochemistry * Extensive experience in all aspects of recombinant molecular techniques including DNA and RNA isolation, PCR, RT-PCR, cDNA library construction, luciferase gene reporter systems, sequencing, site-directed and random mutagenesis. * Strong experience in gel electrophoresis including agarose, SDS-PAGE and Western Blot and immunoprecipitation. * Strong experience in recombinant antibody purification. * Experienced in recombinant protein extraction (GST and His-Tag). * Experienced in protein quantification. Animal models * Experienced in work with rodent (mouse & rat) models. Bioinformatics and Computer Skills * Experienced in protein and DNA sequence search and alignment Vector NTI software. * Experienced in data presentation and statistical analysis with GraphPad Prizm and Ex- cel software.
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