Description
Experienced with CRISPR CAS9 and knockout mice. Purified recombinant proteins including v-src, MAP kinase, MEK and full length nuclear receptors. Experienced with protein expression and purification using Pichia pastoris, E. coli and baculovirus expression systems. Established and maintained primary cell cultures from human, avian, and murine tissues. Experienced in culturing mammalian cell lines. Experienced in molecular biology techniques including transfection, Southern blotting, PCR, plasmid purification, DNA extraction and analysis, and site directed mutatagenesis. Experienced in lipid, inositol phosphate, and protein extraction methods and analyses. Experienced in HPLC, affinity chromatography, and gel filtration. Experienced in immunoblotting, immunoprecipitation, ELISA, and RIA. AWARDS, FELLOWSHIPS, GRANTS T32 Training Fellowship Experienced with protein expression and purification using Pichia pastoris, E. coli and baculovirus expression systems. Established and maintained primary cell cultures from human, avian, and murine tissues. Experienced in culturing mammalian cell lines. Experienced in molecular biology techniques including transfection, Southern blotting, PCR, plasmid purification, DNA extraction and analysis, and site directed mutatagenesis. Experienced in lipid, inositol phosphate, and protein extraction methods and analyses. Experienced in HPLC, affinity chromatography, and gel filtration. Experienced in immunoblotting, immunoprecipitation, ELISA, and RIA. AWARDS, FELLOWSHIPS, GRANTS T32 Training Fellowship
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