Qualification summary * 10+ years' experience in the areas of host pathogen interaction and generation of Knockout in Mycobacterium marinum and M. tuberculosis. * Molecular cloning, RNA extraction and purification, PCR-based analyses, cDNA synthesis, protein expression and purification, Real Time PCR analyses, genomic DNA extraction, Southern blot analysis and basic electron microscopy, Site directed mutagenesis, and complementation studies. * Experience in lipids extraction and thin layer chromatography (TLC) analysis from M. marinum, THP-1 culture, amoeba culture, Raw cells culture, human monocyte-derived macrophages, drug susceptibility testing (category 3 bio-safety facility, South Africa, France and the USA) * Successful track record of carrying out multiple projects from conception through implementation within a given time frame. * Designed and managed multiple research projects simultaneously, led projects independently, delivered lab protocols, 11 scientific articles, and 8 presentations in meetings.

Highlights:

• Realtime
• Test
• Host-pathogen interaction, bacteria, qRT, protein purification, molecular biology, PCR, Western blot
• HTTPs
• Western blot
• English Language
• Focus
• French Language
• Infectious diseases, Mycobacteria, bacteria, protein purification, protein expression, PCR, real time PCR, molecular biology, microbiology, tissue culture, western blot, DNA, RNA, qRT-PCR, RT-PCR, fluorescence microscopy