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SUMMARY____________________________________________________________________ * Biochemical research for the expression, purification, and characterization of key mammalian enzymes. Produced recombinant enzymes in the fermenters and used them for in-vitro experiments. Published data in reputed international journals like PLOS One, Biochemistry & Cell Biology, Vaccine, etc. * Phage display of peptides on the surface of bacteriophage and using it for determining the substrate specificity of proteases like some kallikreins and implantation serine proteinases. Published data in journals like Biological Chemistry & PLOS One. * Successfully optimized & implemented the process for manufacturing of Cephalosporin C (a 3rd generation antibiotic) in the plant having multiple fermenters of 10,000 Liters each and many down-stream processing (DSP) steps including MF, RO, UF, etc. PROFESSIONAL / RESEARCH EXPERIENCE * 2003 - Present Research Associate and Adjunct Faculty, Department of Molecular Biology & Biochemistry, Faculty of Medicine, University of Calgary, Calgary (AB) * Carried out research in the field of implantation serine proteases involved in the implantation and reproduction mainly along with work on the 'expression, characterization, purification and structural proteomics of protein complexes by Mass Spectrometry.' Successful in purifying ISP1 & ISP2 by expressing them in E. coli and purification using FPLC columns for ion exchange, gel filtration and affinity columns. * Bacteriophage display of small peptides on the surface of Lambda bacteriophage and exploiting it for determination of substrate specificity of proteases. Involved in vitro bio-catalysis, FPLC, and Mass Spectrometry. Presented these results in a talk at 'International Proteolysis Society' conference in Santorini, Greece. * Teaching of Master's in Biomedical Technology (MBT) students in the field of 'Biotechnology Business Development' as well as 'Bio-manufacturing and Clinical trials.' Overall at least 200 students would have graduated from the program during these years and many were successfully placed in industry. * 1998 - 2002 Senior Assistant Manager (Process Development) at DSM Anti-Infectives India Ltd. Chandigarh (India) * Developed production processes including down-stream purification process in a large fermentation (10,000 L x 7) facility for the production of Cephalosporin C. Responsible for fermentation process (culture, seed preparation, main fermentation) as well as down stream processing (microfiltration, adsorption chromatography, reverse osmosis, crystallization and vacuum drying) introduced new technologies such as scavenger column (for the removal of excess color and impurities) for improvement in purification. * Involved in the production of Penicillin acylase applied immobilizing technology on acrylonitrile beads after purification using the immobilized enzyme for the bioconversion of Penicillin G into 6-APA and 7-ADCA. * Provided Quality oversight and maintenance (in house) of the batches and all the downstream processing before transferring to Quality control for final testing (as per GMP). Introduced HPLC in-process controls to monitor quality. Reviewed quality of the batches and performed validation (as per GMP) by generating a validation report at the end. Provided the Process validation and optimization of the raw material usage including all the fermentation components. * 1993 - 1998 Assistant Manager (R&D) at MaxGb India Ltd. Chandigarh (India) * Performed R&D work for the fermentation development of Cephalosporin C production at lab-scale and developed the process to industrial scale (3000L). * Optimized downstream processing components of the cephalosporin C production process including microfiltration, adsorption chromatography (1-10 liter glass columns), decolourization chromatography (0.5-2 liter glass columns), reverse osmosis concentration, crystallization, and drying. * Developed several analytical methods including methods for the determination of the content of Cephalosporin-C and the impurities by HPLC. Also developed bio - catalysis process for conversion of Cephalosporin C into 7-ACA as per the technology given by M/S Boehringer Mannheim, Germany.
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