Description
2. Shan Lu's Lab) * Characterized antigen-specific antibodies and B cell responses in various vaccine modalities. * Recombinant protein expression, purification and analysis. * Develop DNA vaccine. Outcomes: * Established B cell Elispot to detect antigen specific memory B cells and plasma cells using mice spleen and bone marrow cells. * Developed new techniques to identify antigen specific B cell using flow cytometry. * Found that optimized 'prime-boost' strategy induced robust HIV gp120 specific B cell and antibody response in mice model. * Presented in professional meetings: 1. DNA priming immunization is more effective than alum in enhancing HIV-1 Env-specific antibody and B cell responses in a mouse model. Keystone Symposia on Molecular and Cellular Biology, HIV Vaccines (X5). Elevated Antigen-specific B cell Responses in Mice Immunized with a DNA Prime-Protein Boost HIV-1 Env Vaccine. DNA Vaccines 2011. (July 12-14 2011 San Diego CA)
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