Description
Professional Summary Lead researcher specialized in Cancer Biology, Molecular Biology, Cell biology and Tissue Fibrosis. Successfully lead two research grant proposals, demonstrated effective interpersonal, written/verbal communication skills and team player with rich publications. Core Qualifications * Cloning, adenovirus and lenti virus * Genetic and epigenetic therapeutic approaches for constructs, siRNA, shRNA, PCR, SDS- fibrosis. PAGE, ELISA, Cell signaling, Cell * Small animal surgeries: Corneal fibrosis (Alkali culture, transfections, stable cell selection, injury) model (rabbit & mouse) kidney capsule western blot and luciferase assays. xenograft techniques, UUO, Urethral obstruction * Experienced biochemist with a broad (UUO), xenograft, etc. knowledge of cancer biology (lung, * Tissue sections and staining techniques (H&E, prostate) and molecular biology (cloning Immunohistochemistry & Immunofluorescence methods). methods. * Familiar with small molecule inhibitors in * Interpretation of results, manuscript and grant cancer treatment and stromal-epithelial or proposal preparation. tumor microenvironment and growth * Experience in cloning Adeno and Lenti virus. factor signaling studies. * Experienced in preparation of IRB and IACUC * In vitro Protein expression, protein protocols. purification by GST and Histidine column, * Excellent computer skills (Microsoft word, excel, GST-pull down assay. PowerPoint, Photo shop and Graphpad. Hands on Expertise * Corneal fibrosis (rabbit and mouse model), epigenetic mechanisms regulating corneal fibrosis, growth factor signaling and adenoviral mediated gene therapy. * Glaucoma filtration surgery induced fibrosis, Therapeutic agents targeting fibrosis. * Anti-fibrotic strategies using nanomedicine based gene therapy. * Advanced research of AR, FoxA1, NFlx protein-protein interaction in Castration Resistant Prostate Cancer (CRPC) (cloning NF1 -CT1, - NT1 deletion constructs and in-vitro protein expression) * Established Transforming growth factor signaling in bladder fibrosis in transgenic mouse models. * PPAR? signaling in urothelial cell biology * Bone marrow mesenchymal cell use in bladder regeneration and fibrosis models * Small molecule inhibitors of TGF? - receptor kinase and HDAC inhibitors and its role in lung cancer treatment strategies. * STRAP interacting protein and explored its interaction by MALDI-TOF and its function in cancer progression * Handling of animals' mice, rat, rabbits and primates 1
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