Summary of Technical Skills: * Demonstrated expertise in generation of antibody fragments (scFv and Fab) for reagent purposes and therapeutical candidates through construction of phage-displayed antibody libraries, and high throughput antibody screening. * Strong experience in the design and construction of bispecific antibody with bi-and tri-scFv formats. * Strong hands-on experience in small and large-scale antibody (scFv and Fab) expression in E. coli and IgG expression in mammalian cell (HEK293 and CHO cell lines) by transition and stable transfection. * Hands-on experience in purification of antibody and protein with affinity system and FPLC (Akta and Bio-Rad NGC). * Strong hands-on experience in characterization of protein by ELISA, SEC, SDS-PAGE, Western Blot, immune-precipitation, and kinetic study with Octet RED96 and BiacoreT200.

Highlights:

• New York
• antibody/ protein engineering
• phage library
• protein expression and purification
• mutagensis
• panning screening
• Construction
• Fab
• Chemistry
• Derivatives
• Research
• Engineering
• Business Intelligence
• COLI