Description
Virologist with 8 years of research experience on multiple viruses (norovirus, adenoviruses, rotaviruses, hepatitis A virus, and ortherovirus). * Experienced in working on variety of mammalian and human cell lines (MA104, MK-LLC2, human cancer cells A549, RAW264, Vero cells, and insect cells). * Experienced in aseptic cell culture with both suspension (E. coli) and adherent mammalian cell lines. * Routinely conducted various assays such as ELISA, plaque assay, functional assay (virus binding assay), primers design, RT-PCR, PCR/qPCR, RNA/DNA sequencing, cell and beads protein binding. RESEARCH SKILLS * Cell biology: culturing different animal and human cells lines (MA104, MK-LLC2, human cancer cells A549, RAW264, Vero cells, and insect cells), confocal microscopy, and immunostaining. * Molecular biology and Biochemistry: PCR, RT -PCR, RT -qPCR, SDS-PAGE, Western blot, RNA extraction, RNA electrophoreses, DNA extraction, ELISA, and sequencing. * Virology: TCID50, plaque assay, virus growth, virus purification, virus sequencing, virus protein analysis, and viruses adaption from environmental and stool samples to cell cultures. * Bacteriology: Aseptic and sterile techniques, bacterial staining, enumeration of bacteria by plating method, membrane filtration, and MPN identification of bacteria by selective medium, API systems, PCR, serotyping antibiotic assay (MIC). * Chemistry: HPLC, standard curve preparation, and data analysis. * Proficient in DNASTAR Lasergene, BLAST, NCBI tools, Primer design softwares, STATA, SPSS, and Microsoft Word, Excel, and PowerPoint.
Work Experience
| COMPANY | POSITION HELD | DATES WORKED |
|---|---|---|
| (Confidential) | Postdoctoral Research Associate, Dept. Of Civil And Environmental | 11/2015 - Present |
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