Well-qualified and technically-proficient Research Scientist with more than five years laboratory experience and excellent academic qualifications. * Substantial experience in sophisticated research techniques and technologies. * Organized, take-charge professional with exceptional follow through abilities and detail orientation able to plan and oversee projects from concept to successful conclusion. * Demonstrated ability to efficiently prioritize a broad range of responsibilities in order to achieve maximum level of operating effectiveness. * Expertise in lab research, data collection/analysis and project management. * Extensive experience working with cross-functional scientific and research teams. TECHNICAL EXPERIENCES Immunology: Immunofluorescence microscopy, Immunoprecipitation, Immunohistochemistry, HLA Typing, Mixed Lymphocyte Culture, Western Blotting and SDS-PAGE, 12 color flow cytometry studies, analysis, interpretation and presentation of multi-color flow cytometry by using biological data analysis tools (FACS DIVA, FlowJo, etc), Prepare SOP for several multi-color flowcytometry panels by following quality control (QC) principles, cytokine intracellular staining, CFSE (Carboxyfluorescein succinimidyl ester) cell proliferation assay, T cell proliferation assay, Isolation of PBMC from Leukapheresis, Preparation and production of dendritic cells (DCs) from human PBMC and mice bone marrow, siRNA knock-down of genes in cell lines, DCs and B lymphocytes, multicolor ELISPOT assay, Prepare SOP for multicolor human ELISPOT assay and apply QC principles, ex vivo expansion of tumor specific T cells and adoptive T cell therapy in murine tumor models. Molecular biology: Plasmid DNA isolation and purification, Genomic DNA and RNA isolation and purification from whole blood, fresh tissue and tissue blocks, PCR, qPCR, PCR-RFLP, PCR-SSCP, ARMS-PCR, DNA fragments cloning in plasmid, DNA transformation, DNA transduction. Cell culture techniques: Culturing and long-term preservation of different adherent and non-adherent cell lines. Establishment of stable cell lines by different transfection methods. Animal skills: Mouse handling, intraperitoneal, intravenous and subcutaneous injection, spleen, thymus and lymph nodes removal, establishing and breeding of mouse cancer models. Working with Viral Vectors: production, amplification, purification and transduction of Adenoviral, Retroviral and Lentiviral vectors in cell lines, Dendritic cells and T cells. Testing immunogenicity, efficacy and safety of viral transduced proteins to use them as novel vaccines. Exosomes: exosomes preparation from cell culture supernatants, isolation by ultracentrifugation and their flow cytometry analysis. Microbiology: Isolation, screening and identification of bacteria and fungi. Antibiotic screening. Hematology: WBC, RBC and Platelet count, microscopic analysis of peripheral blood smear etc.
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