Description
* Accomplished researcher with strong skills & knowledge in various Molecular Biology, Cell Biology & Biochemistry techniques. Research papers published in top professional journals. * Skilled in Circulating Tumor Cell (CTC) capture, Liquid biopsy platform, Biomarker development, Multicolor flow cytometry, Cell based assay development, Immunofluorescence staining of CTCs, Fluorescence in situ hybridization of CTCs, Fluorescence microscopy, Flow cytometry analysis of cell surface marker expression, Immunoassay, Operetta high throughput imaging, Bioview imaging and analysis of FISH slides, Harmony image analysis, Antibody qualification, Growth Factor Bioassays, Assisted Reproductive Technology, Mouse Embryo Genetic Assay, In vitro Maturation Medium, Comparative Genome Hybridization, Next generation sequencing, qPCR, ELISA, Analytical testing * Well versed with MS Office (MS Excel, MS Word & MS PowerPoint). Graphpad Prism, Cell profiler, Image J. GMP, GLP and GDP. EXPERTISE: * Circulating Tumor Cell (CTC) capture * Liquid biopsy platform * Biomarker development * Multicolor flow cytometry * Cell immunofluorescence staining * Fluorescence in situ hybridization * Fluorescence microscopy * Operetta high throughput imaging * Bioview imaging and analysis of FISH slides * Harmony image analysis * Cell based assay development * Immunoassay * Antibody qualification * Growth Factor Bioassays * Assisted Reproductive Technology * Mouse Embryo Genetic Assay * In vitro Maturation Medium * Comparative Genome Hybridization * Next Generation Sequencing * qPCR * ELISA * Agilent Bioanalyzer * Analytical testing * Microsoft Excel, Word and PowerPoint. * Graphpad Prism * Cell profiler * Image J * GMP, GLP and GDP SCIENTIFIC SKILLS: Circulating Tumor Cell Biomarker development, Circulating Tumor Cell (CTC) capture by liquid biopsy platform, Spike in cancer cells to whole blood, 1 mL protocol blood prep, 8 mL protocol blood prep, Immunofluorescence staining of CTCs, Fluorescence in situ hybridization (FISH) of CTCs, Nick Translation Labelling of FISH BAC probes, Development of Breast Cancer FISH detection kit, FSH analysis of patient blood samples, Enrichment of CTCs from whole blood, Myeloid cells depletion from whole blood, PBMC isolation from whole blood, in vitro culture of PBMC, Operetta high throughput imaging of chips after CTC capture, Columbus script analysis of CTC enumeration and white blood cell enumeration, Elution of chips after CTC capture for downstream next generation sequencing analysis, EVOS fluorescent microscope imaging of chips after CTC capture, CX5 high throughput imaging of chips after CTC capture, Liquid biopsy platform capture of fetal cells from maternal blood, Single cell analysis Antibody Qualification Fluorescence Biotin Quantification of antibody, Modified protein Lowry assay of antibody, Titration of primary antibody by flow cytometry, Titration of secondary antibody by flow cytometry, Fluorophore quantification of antibody, Antibody stability test, Multicolor Flow cytometry analysis of cell surface marker expression Cell Culture Small to large scale bioreactors, Process development, In vitro assay development, Primary culture of human airway epithelial cells, Air-liquid interface culture of differentiated human airway epithelial cells, Primary T cell culture, Primary Blood Mononuclear Cell culture, CTLL-2 suspension cell culture, MO7e cell culture, Retina pigment epithelial cell isolation and culture, Primary culture of bovine retinal microvescular cells, mammalian muscle cells, human umbilical vein endothelial cells and cancer cell line culture, generation of stable cell lines Cell Biology Immunohistochemistry, Cell immunofluorescence staining, Phase contrast microscopy, Fluorescence microscopy, Immunocytochemistry, Expression cloning, Metaphase spread preparation to count chromosome numbers and FISH analysis, Preparation and analysis of routine G-, C-and R-banded chromosome, DNA FISH, PNA FISH, LNA FISH, Comparative Genome Hybridization, Cell transfection (Fugene 6 transfection, Fugene HD transfection, Calcium phosphate transient transfection), Cell subcloning, Cell infection by adenovirus and retrovirus, Cell transformation assay, Growth curve analysis, Cell adhesion assay Virology Adenovirus transduction, Retrovirus production, Recombinant protein expression and purification using bacterial and baculovirus systems Biochemistry SDS-PAGE and Western blot, Immunoprecipitation and pull-down assays, Overlay assay, Solid phase binding assay, Receptor binding assay, Autoradiography, Size exclusion chromatography, HPLC, ELISA, Competitive ELISA, Complement activation assay, MSD technology, Sypro ruby protein gel stain, Antibody purification using caprylic acid, Gradient NuPAGE gel preparation, Maltose binding protein purification, Analytical testing Molecular Biology DNA extration and purification, RNA extraction and purification, Miniprep and Maxiprep, NucleoBond BAC 100 extraction of bacterial artificial chromosomes and phage artificial chromosomes, Probe labeling, Fluorescence in situ hybridization, Comparative Genome Analysis, Agarose gel and polyacrylamide gel electrophoresis, Plasmid preparation and purification, DNA transfection, siRNA design and transfection, DNA profile analysis by flow cytometry, 96-well flow cytometry, Fluorescence activated cell sorting (FACS analysis), TUNNEL assay, Southern blot, Northern blot, qPCR and RT-PCR, PCR-SSCP, PCR-RFLP, DNA cloning, Next generation sequencing, TRAP assay, Telomere length analysis, Loss of heterozygosity analysis, Competent cells preparation and transformation Productivity/Bioinformatics Endnote, Adobe Photoshop, Microsoft Office (PowerPoint, Excel, Word), PubMed/Ovid MEDLINE, Genbank, Sequence analysis, PCR primer design, UCSC genome browser, Ensemble, BLAT
Accomplishments
Highlights:
Keywords
- ELISA
- Microsoft Excel
- Microsoft PowerPoint
- Oncology
- pharmaceutical
- Fluorescence in situ hybridization
- Cell based assay
- Multicolor flow cytometry
- Cell culture
- Biomarker development
- Genetics
- Molecular Biology
- Next generation sequencing
- Liquid biopsy
- Microfluidic
- Antibody discovery and qualification
- Primary immune cell culture
- Immunoassay
- Western blot
- Immunohistochemistry
- Immunofluorescence staining
- Biotech
- Cell biology
- Biochemistry
- Immunology
- Small molecule screening
- PCR
- qPCR
- Circulating tumor cell isolation
- Blood prep
- Process development
- Product development
- Bioassay
- Highthroughput imaging
- Highthroughput data analysis
