Description
Areas of Expertise Research and Development: Cloning and Expression of Recombinant Proteins in Multiple Vectors and Purification Strategies, Resolution of Complex Protein Lysates, Protein Chemistry, Fluorescent/Bioluminescent imaging, Assay Development, Perfusion, Batch/Fed-Batch, WAVE Bioreactor Development, Cell Culture, Tissue Extraction, Western Blotting, membrane, cytosolic periplasmic and inclusion body expression, Ammonium Sulfate Precipitation, protein refolding Downstream Manufacturing: Chromatography Packing/ Selection, Bioassay Development and Validation, Monoclonal Antibodies, Enzyme Production, Therapeutic Proteins, Buffer Requirements, characterization of purification intermediates for CMC, Scale-up, endotoxin gel-clot and removal, adventitious viral removal, UF/DF Upstream/Downstream Methods Development: Chromatography: HPLC, FPLC, Gel Filtration (SEC)/IMAC, Protein A/G, IEX/HIC/�KTA FPLC, ELISA Development/Optimization, Theoretical Plates: gravity vs. gradient, DOE (JMP) Quality Control: Protein Lyophilization and Stability Testing, Maintenance and testing of purified intermediates during phases of preparation (e.g. Concentration/ Chemical Modification), Detection of Fragmentation of Proteins and Peptides (SEC, LC - MS), CQA identification (Protein Process) Analysis and Reporting: Root Cause Analysis, Statistical Analysis, Technical Writing, Peer-Reviewed Publication, Reviewer of manuscripts, Quality Assessment, Design of Experiments (JMP) Laboratory Management: Process Optimization, Risk Management, Cost Savings Initiatives, Project Leadership, Technician Supervision, Six Sigma Methodologies, cGMP Batch Records
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