Buck Institute For Age Research & Ucsd Work Values

Daily Duties at Buck Institute For Age Research & Ucsd:

• Investigate the protein biochemistry and function of secretory granules of neuroendocrine chromaffin cells utilizing proteomic approaches - purification of granules, gel electrophoresis, Western blots, microLC-MS/MS, and bioinformatics. • Determine specific cleavage sites by analyzing the primary sequences and potential proteases (prohormone convertases) responsible for the cleavages. Proteomic methods were utilized – purification of granules from adrenal glands, 2D gel electrophoresis, mass spectrometry (MALDI-TOF) & bioinformatics analysis, Edman sequencing & bioinformatics, immunoassays. • Write a postdoctoral research proposal based on the above work to determine the responsible proteases for the processing by biochemical, proteomics, and molecular/cell biological methods. • Investigate the mechanisms of Huntington’s disease (HD) by analyzing HD brain tissues for differential protein expression and elucidating the fragments of mutant huntingtin protein, primary causative agents of the disease. Proteomic approaches were utilized that included biochemical & immunochemical methods (Western blots, IP, IHC), mass spectrometry, differential expression analysis by spot quantitation & immunohistochemical staining and microscopy. • Synthesize new hypotheses drawing from the results of the above projects and from other results in the literature. • Manage and coordinate multiple projects, which involved collaborators from several institutions as the first author investigator of the projects working with the PI.

What they like about Buck Institute For Age Research & Ucsd:

A hiring company that offers alternative approaches to pay, performance bonuses, and/or equity in the firm is very attractive to you. You're generally less interested in working for an organization offering only traditional, salary-based compensation, one with limited opportunity to acquire equity in the firm, or one that does not actively promote skill development. In addition, you are more likely to choose a company that offers a variety of internal career options, with defined career paths. You view the learning of new skills and development of your expertise as key to your career advancement. These aspects of an organization may become even more important to you as you progress in your field, and are especially critical if you change career or occupation.

Tags

Biochemistry, Immunoassays, Oncology, Cell Biology, Neurodegenerative diseases, Proteins, Protein processing, Enzyme assays, Proteases & Peptides, Antibodies & Antigens, Proteomics, Signaling & Therapeutics, Pathobiology, Substrates & Inhibitors, Cancer mechanisms, Neurobiology & Neuroendocrine, FACS, Molecular recognition, Writing & Grants & Editorial, Structure & Function, Laboratory & Manager, Research & Scientist, Mechanisms, Disease pathways, Molecular drug delivery, Proteases, Substrates, Structure, Antibodies, Signaling, Molecular mechanisms, Laboratory Manager, Endocrine, Research Scientist

Skills

Excellent analytical & problem solving skills with cross functional abilities, Multidisciplinary approaches to solving research questions, proteomic approaches to determining biological structure and function, Expertise in protein processing & neuropeptide research, Expertise in proteases, their substrates & inhibitors, assay development, Protein and peptide chemistry/biochemistry/cell biology , Molecular recognition and cell signaling, Highly meticulous experimentalist, Research/lab management, editorial, grants, teaching, Academic and Industry experience

These are some of the questions we asked our climbers about their experiences with Buck Institute For Age Research & Ucsd: