University Of Connecticut Work Values

Daily Duties at University Of Connecticut:

Performed X-ray Crystallography of DHFR from three species. Expressed and purified Vancomycin-resistant enterococcus DHFR, human, and Klebsiella DHFR. Produced crystals of all three varieties. Produced multiple high-resolution diffraction X-ray crystallization data sets. Analyzed ternary crystal structures of DHFR from three species for the purposes of understanding structural variations between these and other species to guide designs of inhibitors for each. This includes characterizing and analyzing molecular differences between these. Performed Sybyl small-molecule docking as well as active site binding characterization using online tools for the purposes of understanding molecular binding site of proteins and biophysical characteristics affecting inhibitor binding. Successfully completed first structure of Klebsiella pneumonaie DHFR (ternary structure with cofactor and inhibitor). Performed IC50 assays with human, vancomycin-resistant enterococcus, and Klebsiella pneumoniae DHFR inhibitors to aid in further inhibitor designs. This is for the purpose of understanding the activity of the proteins, and how it varies under the influence of small molecules with varying chemical characteristics. Managed and trained persons on the in-house X-ray beamline. Instructed, demonstrated, and advised two students’ biochemical and X-ray crystallography techniques for PharmD student projects. Served as guest lecturer for course, “Structure and Function of Biological Macromolecules.” As guest lecturer, described general X-ray crystallography procedures, beginning at setting up crystallization trials. Reviewed X-ray facility instrumentation and initial computational methods for solving structures. Served as assistant to ECE Professors’ Training Courses (2 sessions). During this, I reviewed practical methods in X-ray crystallography.

What they like about University Of Connecticut:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

Research and Development, microbiology, molecular biology, biochemistry, protein purification, qPCR, X-ray crystallography, cryo-EM, homologous recombination, recombinant virus, mammalian cell transfection, virology, protease activity assay, TRITC fluorescence, fluorescence-based assay

Skills

Plasmid and genomic DNA purification, Supervision of small group (2 subordinates) , PCR, qPCR, Reverse transcription PCR, Cloning, mutagenesis, DNA electrophoresis, DNA/RNA sequence analysis, Homologous recombination, Viral propagation and titration, RNA dependent RNA Polymerase assay, neutralization assay, Mammalian cell culture, Transfection of mammalian cells, Protein purification (affinity, size exclusion, reverse phase, etc.), SDS Page gels, Agarose gels, Western Blotting, Protease activity assay, IC50 assay, Protein active site analysis, Structure based drug design, X-ray crystallography, Negative stain and immuno-labeling electron microscopy, Fluorescence microscopy, cryo-tomography data reconstruction, Segmentation of cryo-tomography data, Bacterial protein expression, Bacterial protein expression

These are some of the questions we asked our climbers about their experiences with University Of Connecticut: