Emory University Work Values

Daily Duties at Emory University:

Conducted In vitro assembly of respiratory syncytial virus matrix protein lattice. Aimed to determine arrangement of Measles Virus (MeV) glycoproteins in mature virions. Performed cryo-tomography reconstructions of RSV-infected cells to understand protein arrangements and changes at early-stages of infection. Purified respiratory syncytial virus matrix protein from E. coli protein expression system and produced liposomes from Avanti lipid to understand protein behavior within context of viral membrane environment. Designed and executed reactions for respiratory syncytial virus matrix lattice assembly. Evaluated assembly reactions by negative staining and viewing on JEOL1400 microscope. Grew HeLa cells on quantifoil grids and infected them successfully with Measles virus strains Measles-HcHisTagFlag and Measles-GFP. Evaluated infection of HeLa cells with Measles-GFP virus via fluorescence. Cryo-plunged froze infected HeLa cells on grids for cryo-tomography data collection. Processed cryotomography data resulting from above mentioned Measles grids as well as from other projects in the lab using IMOD software. Processed cryo-tomography data to support projects of other laboratory members. Carried out TCID50 assays for Measles tittering.

What they like about Emory University:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

Research and Development, microbiology, molecular biology, biochemistry, protein purification, qPCR, X-ray crystallography, cryo-EM, homologous recombination, recombinant virus, mammalian cell transfection, virology, protease activity assay, TRITC fluorescence, fluorescence-based assay

Skills

Plasmid and genomic DNA purification, Supervision of small group (2 subordinates) , PCR, qPCR, Reverse transcription PCR, Cloning, mutagenesis, DNA electrophoresis, DNA/RNA sequence analysis, Homologous recombination, Viral propagation and titration, RNA dependent RNA Polymerase assay, neutralization assay, Mammalian cell culture, Transfection of mammalian cells, Protein purification (affinity, size exclusion, reverse phase, etc.), SDS Page gels, Agarose gels, Western Blotting, Protease activity assay, IC50 assay, Protein active site analysis, Structure based drug design, X-ray crystallography, Negative stain and immuno-labeling electron microscopy, Fluorescence microscopy, cryo-tomography data reconstruction, Segmentation of cryo-tomography data, Bacterial protein expression, Bacterial protein expression

These are some of the questions we asked our climbers about their experiences with Emory University: