Welgen, Inc. Work Values

Daily Duties at Welgen, Inc.:

Worked with yeast expression system: constructed recombinant pDNAs and yeast strains, performed pilot fermentations in flasks, analysed protein expression by SDS-PAGE, coomassie and silver staining, estimated antibacterial activity by plate assays, determined MIC in 96-well plates. As the Research Scientist worked with the literature, discussed optimization of experimental conditions with president and team members, did experiments according to established optimized protocols and organized results to deliver the product as soon as possible.

What they like about Welgen, Inc.:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

Cell Biology, Genetics, Microbiology, bacteria, yeast, cancer, cell line, fluorescence microscopy, confocal microscopy, strain engineering, enzyme activity assays, microbiological tests, pilot fermentation in flasks, team leadership/supervision, scientific presentations, manuscript preparation, recombinant DNA, protein expression, trafficking

Skills

yeast culturing, bacteria culturing, mammalian cell line culturing, transformation/transfection, immunocytochemistry, DAPI staining, fluorescence and confocal microscopy, antibacterial plate assays, MIC determination, yeast mating and sporulation, UV and insertional mutagenesis, screening and mutant isolation, enzymatic assays in yeast colonies, protein expression, protein amount and activity measurement, protein-protein interactions, yeast two-hybrid system, co-immunoprecipitation, crosslinking, SDS-PAGE, native PAGE, coomassie staining, silver staining, Western Blot, plasmid and genomic DNA isolation, yeast genomic DNA library construction, cloning and mapping, PCR, site-directed mutagenesis, sample preparation for DNA sequencing, Southern Blot, bacteria freezing, yeast freezing, mammalian cell line freezing, DNA sequence analysis, protein sequence analysis, BLAST, multiple sequence alignments, PubMed search, Windows, MacOS, Microsoft Office, Adobe Photoshop, Clone Manager, Gel Pro, Image J, ABBYY Fine Reader, ZEN, PyMolWin, Team Leadership/Supervision, Scientific presentations, Manuscript preparation, Lab notebook organization

These are some of the questions we asked our climbers about their experiences with Welgen, Inc.: