Johns Hopkins University, Brady Urological Labs Work Values

Daily Duties at Johns Hopkins University, Brady Urological Labs:

  Productively performed methods in Molecular Cloning: Aseptic Techniques in Biological Safety Cabinet, Cell-Culture, Isolation of Nucleic acids, SDS-PAGE and Agarose gel-electrophoresis, PCR, RT-PCR, & qPCR. Cloned plasmids carrying genes of interest to produce Recombinant Adenoviruses. Manipulated bacterial, mammalian cell-lines for altering Gene-expression in cells.  Analyzed Proteins on Isolation, Quantitation and Western blot.  Performed cell-based functionality assays and statistically analyzed the data generated.  Coached new fellows and students in developing lab techniques to achieve individual project goals.  Gained familiarity with Mice sub-cutaneous tumour implantation, surgeries and Luciferin Imaging.  Diligently maintained safety and quality standards. Coordinated preventative maintenance on existing and new lab equipment, including routine calibration. Monitored and maintained inventory of lab & research supplies, using the on-line SAP / SRM requisitioner. Received & shipped materials from various JHU affiliations both in the US & internationally as per the DOT / IATA specifications.  Operated & maintained lab equipments such as PCR, Real-time qPCR Thermalcyclers (ICycler & Taqman), Fluorimeter, Luminometer & Liquid-Scintillation counter, Gel-imager, Centrifuges, pH-meter, Analytical balance, Spectrophotometer, Light and Fluorescent Microscopes, Biological Safety Cabinet, Incubators, Refrigerators, and Freezers.  Used the MESA electronic system to monitor Freezer & Incubator alarms.  Developed and maintained strong and clear communication and rapport with lab personnel. Interacted with vendors for quotations, special pricing and product related technical questions.  Cultivated knowledge of Microsoft office (MS word,Excel, Power point) and proficiency in VectorNTI Software. Carried out tasks in connecting instruments with computers, downloading and installing software, data entry and retrieval.

What they like about Johns Hopkins University, Brady Urological Labs:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Molecular Biologist

Skills

Aseptic Techniques, Cell-Culture, Cell & Virus banking, Virus Purification by Dialysis & kits, Molecular Biology, SDS-PAGE, Agarose Gel Electrophoresis, DNA & RNA Purfication & Quantitation, Gene & miRNA Expression Analysis, PCR, Real-time RT-qPCR, ELISA,, Cell-based experiments, cGMP, GLP, SAP, Maintain inventory of materials, Microsoft office, Excel, MS word, Power point, Statistical Data Analysis, Install software & trouble-shoot on Instruments, Write & Follow SOPs, Quality control, Lab Safety protocols,

These are some of the questions we asked our climbers about their experiences with Johns Hopkins University, Brady Urological Labs: