Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md Work Values

Daily Duties at Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md:

• Supervision of anti-tumor polynucleotide vaccine (PNV) development project • Development of a technique for separating/purifying CCC plasmid DNA mixtures • Plasmid DNA isolation, purification and measurement • Continuous elution electrophoresis • Tumor regression studies (Sprague Dawley rats) • Carried out 2-D SDS PAGE project focused on the regulation of proteins by thymidylate synthase (TS) • Cell culture • 2D SDS-PAGE • Generation of peripheral blood monocyte-derived dendritic cells from NIH Lecopacks

What they like about Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Management, PMP Certification, Scientist, Biologist, Clinical, Biomarker, Biomarker Discovery, Proteomics, Mass spectrometry, LC-MS-MS

Skills

• Management, implementation and execution of large-scale clinical proteomic and biomarker discovery projects: cancer, Barrett's esophagus, SIV, traumatic brain injury, etc., Experimental study design, Clinical sample preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS),  Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., • Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, • Southern blotting, Telomere restriction fragment analysis, • Plasmid DNA isolation, purification and measurement, • Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md: