Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md Work Values

Daily Duties at Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md:

• Supervision of anti-tumor polynucleotide vaccine (PNV) development project • Development of a technique for separating/purifying CCC plasmid DNA mixtures • Plasmid DNA isolation, purification and measurement • Continuous elution electrophoresis • Tumor regression studies (Sprague Dawley rats) • Carried out 2-D SDS PAGE project focused on the regulation of proteins by thymidylate synthase (TS) • Cell culture • 2D SDS-PAGE • Generation of peripheral blood monocyte-derived dendritic cells from NIH Lecopacks

What they like about Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md:

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Tags

Management, PMP Certification, Scientist, Biologist, Clinical, Biomarker, Biomarker Discovery, Proteomics, Mass spectrometry, LC-MS-MS

Skills

• Management, implementation and execution of large-scale clinical proteomic and biomarker discovery projects: cancer, Barrett's esophagus, SIV, traumatic brain injury, etc., Experimental study design, Clinical sample preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS),  Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., • Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, • Southern blotting, Telomere restriction fragment analysis, • Plasmid DNA isolation, purification and measurement, • Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Immunotherapy Laboratory, Saic, National Cancer Institute (Nci), Frederick, Md: