Cystic Fibrosis Research Center, Children Hospital Of Pittsburgh Work Values

Daily Duties at Cystic Fibrosis Research Center, Children Hospital Of Pittsburgh:

• Establish the in vitro study system for CFTR biogenesis and SUMOylation by purifying pathway proteins (wt and mutants) with enzyme activities • Vector construction, Protein overexpression, purification, validation and characterization

What they like about Cystic Fibrosis Research Center, Children Hospital Of Pittsburgh:

The social vibrance of a hiring firm is very important to you. Your ability to make and maintain friendships there is a critical part of your decision. You would likely be dissatisfied with a workplace that is quiet, cold, or otherwise not particularly social. When you investigate a new hiring company, ask recruiters, managers, and potential co-workers about the social life and opportunities there. This is especially important when you are relocating; moving dramatically alters your social sphere both inside and outside the workplace.

Tags

Maryland, Molecular biology, Microbiology, NGS, Protein purfication , Matlab, Java, Nextgene, Molecular Biologist and Microbiologist, NGS scientist, Medical Technologist (MLT)

Skills

Cell culture, fermenter culture, mammalian cells culture, plasmid extraction and construction, DNA/RNA operation, vector construction, transfection and transformation, Vector validation, single nucleotide mutagenesis, Protein overexpression and purification, ELISA, Western blotting, fluorescence based assays, pulse-chase endocytosis tracking, Flowcytometry, Confocal Imaging, fluorescence and phase contrast microscope, SEM, NGS, Ion Torrent PGM, C, matlab, Java, Image J, Nextgene, Prism, library preparation and sample barcoding

These are some of the questions we asked our climbers about their experiences with Cystic Fibrosis Research Center, Children Hospital Of Pittsburgh: