Columbia University Work Values

Daily Duties at Columbia University:

Decommission of a catalytic enzyme to yield an antibody for RDX chemistries Designed, generated, and identified promising mutants of alcohol dehydrogenase imbued with engineered affinity toward royal demolition explosive (RDX) chemistries

What they like about Columbia University:

A hiring company that offers alternative approaches to pay, performance bonuses, and/or equity in the firm is very attractive to you. You're generally less interested in working for an organization offering only traditional, salary-based compensation, one with limited opportunity to acquire equity in the firm, or one that does not actively promote skill development. In addition, you are more likely to choose a company that offers a variety of internal career options, with defined career paths. You view the learning of new skills and development of your expertise as key to your career advancement. These aspects of an organization may become even more important to you as you progress in your field, and are especially critical if you change career or occupation.

Tags

Molecular Biology, Scientist, Chemist, Protein engineer

Skills

Protein expression both with natural and unnatural amino acids and Click Chemistry, Fast protein liquid chromatography (FPLC), High Performance Liquid chromatography (HPLC), Size Exclusion Chromatography (SEC), affinity (Ni2+ and Co2+), cation/ anion-exchangechromatography, Q-Column, factor Xa cleavage and desalting, Enzyme Kinetic Assays, Protein modeling and docking simulations, Gel electrophoresis quantitative staining (ruby, silver staining, Western blotting), Microrheology and Dynamic Mechanical Analysis (DMA), UV-Vis/ Fluorescence spectroscopy, Scanning Electron microscopy (SEM) and confocal microscopy, Dynamic light scattering (DLS) and Zeta Potential, Gas Chromatography-Mass Spectrometry (GC-MS), MALDI-TOF/ TOF MS, Circular Dichroism (CD), Enzyme-linked Immunosorbent Assay (ELISA), Differential Scanning Calorimetry (DSC), Isothermal Calorimetry (ITC)), Thermal Gravimetric Analysis (TGA), DNA library construction, Cloning (Topo, T4 Ligase, Gibson and In-Fusion), Ribosome display and Phage display (high throughput screening/ selection), Molecular Biology PCR (Assembly, Nested PCR, Single/ multi-site SDM, Error prone PCR, DNA shuffling and RT-PCR), RNA recovery and Transfections, In Vitro Transcription and Translation

These are some of the questions we asked our climbers about their experiences with Columbia University: