Immunotherapy Laboratory, Saic, National Cancer Institute (Nci) At Frederick, Frederick, Md Work Values

Daily Duties at Immunotherapy Laboratory, Saic, National Cancer Institute (Nci) At Frederick, Frederick, Md:

Supervision of anti-tumor polynucleotide vaccine (PNV) development project Development of a technique for separating/purifying CCC plasmid DNA mixtures Prieto D, Harvey LK and Nelson EL. 2000. Separation and purification of plasmid mixtures by continuous elution electrophoresis. Biotechniques. 26:1204-1206.  Plasmid DNA isolation, purification and measurement  Continuous elution electrophoresis  Tumor regression studies (Sprague Dawley rats) Carried out 2-D SDS PAGE project focused on the regulation of proteins by thymidylate synthase (TS)  Cell culture  2D SDS-PAGE Generation of peripheral blood monocyte-derived dendritic cells from NIH Leucopacks Nelson EL, Strobl S, Subleski J, Prieto D et al. 1999. Cycling of human dendritic cell effector phenotypes in response to TNF-: modification of the current ‘maturation’ paradigm and implications for in vivo immunoregulation. FASEB. 13:2021-2030

What they like about Immunotherapy Laboratory, Saic, National Cancer Institute (Nci) At Frederick, Frederick, Md:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Scientist, Clinical, Proteomics, Biomarker, Protein/Peptide, Mass Spectrometry, LC-MS, SDS-PAGE, Microsoft Office Word, Microsoft Office PowerPoint, Microsoft Office Excel

Skills

Mass Spectrometry, LC-MS, Clinical Sample Preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Strong cation exchange (SCX) chromatography, Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Sci, Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, Southern blotting, Telomere restriction fragment analysis, Plasmid DNA isolation, purification and measurement, Continuous elution electrophoresis, Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Immunotherapy Laboratory, Saic, National Cancer Institute (Nci) At Frederick, Frederick, Md: