Laboratory Of Immunopathogenesis And Bioinformatics, Saic, National Cancer Institute (Nci) At Frederick Work Values

Daily Duties at Laboratory Of Immunopathogenesis And Bioinformatics, Saic, National Cancer Institute (Nci) At Frederick:

Development and optimization of telemere length assay Completion of chronic lymphocytic leukemia (CLL) and HIV Projects Ishibe N, Prieto D, Hosack D, Lempicki R et al. 2002. Telomere length and heavy chain mutation status in familial CLL. Leukemia Res. 26:791-794. Natarajan VR, Lempicki RA, Sereti I, Badralmaa Y, Adelsberger JW, Metcalf JA, Prieto DA et al. 2002. Increased peripheral expansion of naive CD4+ T cells in vivo after IL-2 treatment of patients with HIV infection. Proc. Natl. Acad. Sci. USA. 99:10712-10717.  Genomic DNA isolation, assessment and restriction digestion  Pulsed-field gel electrophoresis  Southern blotting  Telomere restriction fragment analysis

What they like about Laboratory Of Immunopathogenesis And Bioinformatics, Saic, National Cancer Institute (Nci) At Frederick:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Scientist, Clinical, Proteomics, Biomarker, Protein/Peptide, Mass Spectrometry, LC-MS, SDS-PAGE, Microsoft Office Word, Microsoft Office PowerPoint, Microsoft Office Excel

Skills

Mass Spectrometry, LC-MS, Clinical Sample Preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Strong cation exchange (SCX) chromatography, Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Sci, Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, Southern blotting, Telomere restriction fragment analysis, Plasmid DNA isolation, purification and measurement, Continuous elution electrophoresis, Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Laboratory Of Immunopathogenesis And Bioinformatics, Saic, National Cancer Institute (Nci) At Frederick: