Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick) Work Values

Daily Duties at Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):

Development of an immunodepletion method for the removal of highly abundant proteins from clinical specimens, i.e. fresh frozen tissue Prieto DA, Johann DJ, Wei BR, Ye X, et al. 2014. Mass spectrometry in cancer biomarker research: a case for immunodepletion of abundant blood-derived proteins from clinical tissue specimens. Biomark. Med. 8(2):269-86 Execution of large-scale clinical proteomic and biomarker discovery projects: cancer, SIV, traumatic brain injury, etc.  Clinical sample preparation  Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc.  Strong cation exchange (SCX) chromatography  Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Scientific mass spectrometers (LTQ, LTQ Velos, LTQ Orbitrap XL, LTQ-FT, Q Exactive and Orbitrap Fusion)  Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc. Spearheaded organization of the Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF MS) technology within the laboratory (2002-2007)

What they like about Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):

A hiring company that offers alternative approaches to pay, performance bonuses, and/or equity in the firm is very attractive to you. You're generally less interested in working for an organization offering only traditional, salary-based compensation, one with limited opportunity to acquire equity in the firm, or one that does not actively promote skill development. In addition, you are more likely to choose a company that offers a variety of internal career options, with defined career paths. You view the learning of new skills and development of your expertise as key to your career advancement. These aspects of an organization may become even more important to you as you progress in your field, and are especially critical if you change career or occupation.

Tags

Scientist, Clinical, Proteomics, Biomarker, Protein/Peptide, Mass Spectrometry, LC-MS, SDS-PAGE, Microsoft Office Word, Microsoft Office PowerPoint, Microsoft Office Excel

Skills

Mass Spectrometry, LC-MS, Clinical Sample Preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Strong cation exchange (SCX) chromatography, Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Sci, Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, Southern blotting, Telomere restriction fragment analysis, Plasmid DNA isolation, purification and measurement, Continuous elution electrophoresis, Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):