Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick) Work Values

Daily Duties at Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):

Development of an immunodepletion method for the removal of highly abundant proteins from clinical specimens, i.e. fresh frozen tissue Prieto DA, Johann DJ, Wei BR, Ye X, et al. 2014. Mass spectrometry in cancer biomarker research: a case for immunodepletion of abundant blood-derived proteins from clinical tissue specimens. Biomark. Med. 8(2):269-86 Execution of large-scale clinical proteomic and biomarker discovery projects: cancer, SIV, traumatic brain injury, etc.  Clinical sample preparation  Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc.  Strong cation exchange (SCX) chromatography  Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Scientific mass spectrometers (LTQ, LTQ Velos, LTQ Orbitrap XL, LTQ-FT, Q Exactive and Orbitrap Fusion)  Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc. Spearheaded organization of the Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF MS) technology within the laboratory (2002-2007)

What they like about Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Scientist, Clinical, Proteomics, Biomarker, Protein/Peptide, Mass Spectrometry, LC-MS, SDS-PAGE, Microsoft Office Word, Microsoft Office PowerPoint, Microsoft Office Excel

Skills

Mass Spectrometry, LC-MS, Clinical Sample Preparation, Sample preparation for mass spectrometry: SDS-PAGE, in-gel and in-solution digestions, solid phase extraction (SPE), etc., Strong cation exchange (SCX) chromatography, Protein identification using microcapillary reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS): Thermo Scientific EASY-nLC nano-flow liquid chromatography systems, Agilent 1100, 1200 & 1260 HPLC systems coupled online to Thermo Sci, Bioinformatics: Bioworks, Xcalibur, TurboSequest & Mascot database searching, Proteome Discoverer, ProToPiQ, etc., Genomic DNA isolation, assessment and restriction digestion, Pulsed-field gel electrophoresis, Southern blotting, Telomere restriction fragment analysis, Plasmid DNA isolation, purification and measurement, Continuous elution electrophoresis, Cell culture, 2D SDS-PAGE

These are some of the questions we asked our climbers about their experiences with Protein Characterization Laboratory, Leidos Biomedical Research, Inc. (Formerly Saic), Frederick National Laboratory For Cancer Research (Formerly Nci Frederick):