Suny At Buffalo, School Of Medicine And Biomedical Work Values

Daily Duties at Suny At Buffalo, School Of Medicine And Biomedical:

Development, characterization and conduct of general cloning, Total RNA and mRNA preparation, southern and northern hybridization, screening cDNA library, SDS-PAGE, western blot, immunohistochemistry, ELISA, primary neuro- cell culture (bovine adrenal medullar chromaffin cell) and continuous cells (TE671 cell, PC-12 cell), , transected target gene into mammalian cell, publish scientific articles in peer-review journals, Training graduate students in lab.

What they like about Suny At Buffalo, School Of Medicine And Biomedical:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Molecular biology, cell biology, virology, immunology, protein expression and purification, cell culture, real time QPCR, ELISA, Protein Chemistry

Skills

• Molecular biology techniques: general cloning, PCR, Real-time QPCR (both SYBR Green and Taqmen based), siRNA knockdown techniques, DNA and RNA sequencing and analysis, site directed mutagenesis, southern and northern hybridization, screening cDNA libr, • Virology techniques: growing and developing virus in cell culture, virus plaque assay, virus neutralization and complement fixation assay, virus isolation and purification by CsCl gradients techniques, construction of recombinant viral vectors, co-t, • Immunology ,protein chemistry and protein purification techniques: antigen preparation, antibody purification, animal immunization, ELISA, SDS-PAGE, western blot, immunofluorescence, immuno-precipitation, His-tagging metal-chelate affinity chromatogra, • Cell culture skills: primary cells (primary monkey kidney cell, bovine adrenal medullar chromaffin cell, human umbilical vein endothelial cell ), semi-continuous diploid cells ( human diploid fibroblasts cell ), and continuous cells ( Vero cell, Hep-, • Promoter and transcription factor analysis: promoter region mutation, reporter gene assay (firefly luciferase, GFP), electrophoresis mobility shift assays (EMSA), and DNase I Footprint, • Bioinformatics skills: NCBI online bio informatics tools (GeneBank and BLAST family of programs), sequence analysis tools, protein analysis software (Cn3D4.1, SWISS-MODEL).

These are some of the questions we asked our climbers about their experiences with Suny At Buffalo, School Of Medicine And Biomedical: