University Of Rochester School Of Medicine And Dentistry Work Values

Daily Duties at University Of Rochester School Of Medicine And Dentistry:

Development, conduct and implementation of site directed mutagenesis, expression vector design, and recombinant protein expression and purification with his-tagging metal-chelate affinity chromatography and HPLC. Antibody purification, protein-protein binding assays. . Independently analyze and interpret experimental data, present experimental results at department meetings, publishes in peer-­reviewed journals.Training medical students.

What they like about University Of Rochester School Of Medicine And Dentistry:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

Molecular biology, cell biology, virology, immunology, protein expression and purification, cell culture, real time QPCR, ELISA, Protein Chemistry

Skills

• Molecular biology techniques: general cloning, PCR, Real-time QPCR (both SYBR Green and Taqmen based), siRNA knockdown techniques, DNA and RNA sequencing and analysis, site directed mutagenesis, southern and northern hybridization, screening cDNA libr, • Virology techniques: growing and developing virus in cell culture, virus plaque assay, virus neutralization and complement fixation assay, virus isolation and purification by CsCl gradients techniques, construction of recombinant viral vectors, co-t, • Immunology ,protein chemistry and protein purification techniques: antigen preparation, antibody purification, animal immunization, ELISA, SDS-PAGE, western blot, immunofluorescence, immuno-precipitation, His-tagging metal-chelate affinity chromatogra, • Cell culture skills: primary cells (primary monkey kidney cell, bovine adrenal medullar chromaffin cell, human umbilical vein endothelial cell ), semi-continuous diploid cells ( human diploid fibroblasts cell ), and continuous cells ( Vero cell, Hep-, • Promoter and transcription factor analysis: promoter region mutation, reporter gene assay (firefly luciferase, GFP), electrophoresis mobility shift assays (EMSA), and DNase I Footprint, • Bioinformatics skills: NCBI online bio informatics tools (GeneBank and BLAST family of programs), sequence analysis tools, protein analysis software (Cn3D4.1, SWISS-MODEL).

These are some of the questions we asked our climbers about their experiences with University Of Rochester School Of Medicine And Dentistry: