Biocare Medical Work Values

Daily Duties at Biocare Medical:

Conjugated HRP and Antibody with Periodate activation or with S-HyNic together with S-4FB activation. Fractionation [AKTA PRIME and Gravity columns (Superdex S200; IDA-Ni-charged EDTA gradient)], Characterized (Bradford; end point and kinetic ELISA, HPLC, SDS-PAGE) of conjugates, separation of fractions MW 250-500kD. Improved GLP practice to transfer to GMP. Evaluated Tris-Acetate PAGE for High MW Proteins. Organized proper storage and usage of organic solvents and hygroscopic reagents. Conducted and wrought suggestions for SOP in GMP environment. Introduced TFF system “Spectrum” for separation conjugates of necessary MW range. Established SOPs.

What they like about Biocare Medical:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

Protein purification from mammal cells, tissue, E Coli, , Protein analyses, Protein Conjugation, Developing Immune assays, MAB/FAB Small MW molecular Screening, Phage display screening, Cell culture, transfection, protein producing, San Diego Relocation

Skills

Protein Purification methods: Salt precipitation/solubility,UltraFiltr., gradient centrifugation, column chromatography at normal and higher pressure: SEC, IEX, HP, RP,Hep., HA, IMEC, protA/G, Lectin, Affinity, AKTA-(Express, FPLC, Prime, Explorer); FPLC , Protein characterization methods: PAGE/Western, IEF, 2-D PAGE (Coomassie, Fluorescent, Silver, ECL), HPLC, CD, SPIFE, UV/Vis, Enzymatic assays, LAL,OCTET, FACS, 96/384 well color/fluorescent readers/scanners. , Immunochemical methods: Quantitative sandwich and competitive ELISAs, Dot blotting, immune precipitation, obtaining MABs, epitope binning analysis, affinity EC50. , DNA manipulation: DNA-fragments gel purification, DNA fragment mobility shift assay, digestion, Ligation, Plasmid DNA purification, Transformation, Agarose and PAGE separation of DNA restrictase or PCR products, PCR directed Site mutagenesis, mRNA purific, Cell Culture: Maintaining of mammalian-, insect- and fish- cells in adherent or suspended form. Transient transfection with Ca-Ph, Lpf, Fugen, PEI; infection with baculoviruses and Phages. Creating transfected cell lines. Fusion. Obtaining Hybridomas. , Cell assays: viability assay, fluorescent/visual microscopy, Cyto-staining, FACS, Cell FIA, Cell RIA., Protein conjugation of IgG, FABs, target proteins with Biotin, AP, HRP, fluorescent dyes, I-125, Protein-protein interaction: OCTET, Shift assays: HPLC, native PAGE; ELISA. ELISPOT, HTP instruments: OKTET, EPMotion5075, NEMESIS 7200 immune-Histo-Stain Robot, 20x96 well plate washer, 1-10ml Manufacturing Dispenser. , Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, PRIZM5, ImageQuant, EPBlue.

These are some of the questions we asked our climbers about their experiences with Biocare Medical: