Stanford University/Ucsf Work Values

Daily Duties at Stanford University/Ucsf:

To develop and conduct producing of recombinant Netrin-1 and other proteins, antibodies and reactives. Support work of Post Docs, associates and collaborators in the lab.

What they like about Stanford University/Ucsf:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Protein purification from mammal cells, tissue, E Coli, , Protein analyses, Protein Conjugation, Developing Immune assays, MAB/FAB Small MW molecular Screening, Phage display screening, Cell culture, transfection, protein producing, San Diego Relocation

Skills

Protein Purification methods: Salt precipitation/solubility,UltraFiltr., gradient centrifugation, column chromatography at normal and higher pressure: SEC, IEX, HP, RP,Hep., HA, IMEC, protA/G, Lectin, Affinity, AKTA-(Express, FPLC, Prime, Explorer); FPLC , Protein characterization methods: PAGE/Western, IEF, 2-D PAGE (Coomassie, Fluorescent, Silver, ECL), HPLC, CD, SPIFE, UV/Vis, Enzymatic assays, LAL,OCTET, FACS, 96/384 well color/fluorescent readers/scanners. , Immunochemical methods: Quantitative sandwich and competitive ELISAs, Dot blotting, immune precipitation, obtaining MABs, epitope binning analysis, affinity EC50. , DNA manipulation: DNA-fragments gel purification, DNA fragment mobility shift assay, digestion, Ligation, Plasmid DNA purification, Transformation, Agarose and PAGE separation of DNA restrictase or PCR products, PCR directed Site mutagenesis, mRNA purific, Cell Culture: Maintaining of mammalian-, insect- and fish- cells in adherent or suspended form. Transient transfection with Ca-Ph, Lpf, Fugen, PEI; infection with baculoviruses and Phages. Creating transfected cell lines. Fusion. Obtaining Hybridomas. , Cell assays: viability assay, fluorescent/visual microscopy, Cyto-staining, FACS, Cell FIA, Cell RIA., Protein conjugation of IgG, FABs, target proteins with Biotin, AP, HRP, fluorescent dyes, I-125, Protein-protein interaction: OCTET, Shift assays: HPLC, native PAGE; ELISA. ELISPOT, HTP instruments: OKTET, EPMotion5075, NEMESIS 7200 immune-Histo-Stain Robot, 20x96 well plate washer, 1-10ml Manufacturing Dispenser. , Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, PRIZM5, ImageQuant, EPBlue.

These are some of the questions we asked our climbers about their experiences with Stanford University/Ucsf: