Ucsf Work Values

Daily Duties at Ucsf:

Purification of anti-peptide Antibody. Demonstration of its specificity. Immune-precipitation of CNG receptors from Rod and Cone cells.

What they like about Ucsf:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

Protein purification from mammal cells, tissue, E Coli, , Protein analyses, Protein Conjugation, Developing Immune assays, MAB/FAB Small MW molecular Screening, Phage display screening, Cell culture, transfection, protein producing, San Diego Relocation

Skills

Protein Purification methods: Salt precipitation/solubility,UltraFiltr., gradient centrifugation, column chromatography at normal and higher pressure: SEC, IEX, HP, RP,Hep., HA, IMEC, protA/G, Lectin, Affinity, AKTA-(Express, FPLC, Prime, Explorer); FPLC , Protein characterization methods: PAGE/Western, IEF, 2-D PAGE (Coomassie, Fluorescent, Silver, ECL), HPLC, CD, SPIFE, UV/Vis, Enzymatic assays, LAL,OCTET, FACS, 96/384 well color/fluorescent readers/scanners. , Immunochemical methods: Quantitative sandwich and competitive ELISAs, Dot blotting, immune precipitation, obtaining MABs, epitope binning analysis, affinity EC50. , DNA manipulation: DNA-fragments gel purification, DNA fragment mobility shift assay, digestion, Ligation, Plasmid DNA purification, Transformation, Agarose and PAGE separation of DNA restrictase or PCR products, PCR directed Site mutagenesis, mRNA purific, Cell Culture: Maintaining of mammalian-, insect- and fish- cells in adherent or suspended form. Transient transfection with Ca-Ph, Lpf, Fugen, PEI; infection with baculoviruses and Phages. Creating transfected cell lines. Fusion. Obtaining Hybridomas. , Cell assays: viability assay, fluorescent/visual microscopy, Cyto-staining, FACS, Cell FIA, Cell RIA., Protein conjugation of IgG, FABs, target proteins with Biotin, AP, HRP, fluorescent dyes, I-125, Protein-protein interaction: OCTET, Shift assays: HPLC, native PAGE; ELISA. ELISPOT, HTP instruments: OKTET, EPMotion5075, NEMESIS 7200 immune-Histo-Stain Robot, 20x96 well plate washer, 1-10ml Manufacturing Dispenser. , Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, PRIZM5, ImageQuant, EPBlue.

These are some of the questions we asked our climbers about their experiences with Ucsf: