Sea Lane Biotechnologies Work Values

Daily Duties at Sea Lane Biotechnologies:

FABs screening, producing, purification, characterisation. Developing Immunoassays and purification methods. Writing SOPs.

What they like about Sea Lane Biotechnologies:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Protein purification from mammal cells, tissue, E Coli, , Protein analyses, Protein Conjugation, Developing Immune assays, MAB/FAB Small MW molecular Screening, Phage display screening, Cell culture, transfection, protein producing, San Diego Relocation

Skills

Protein Purification methods: Salt precipitation/solubility,UltraFiltr., gradient centrifugation, column chromatography at normal and higher pressure: SEC, IEX, HP, RP,Hep., HA, IMEC, protA/G, Lectin, Affinity, AKTA-(Express, FPLC, Prime, Explorer); FPLC , Protein characterization methods: PAGE/Western, IEF, 2-D PAGE (Coomassie, Fluorescent, Silver, ECL), HPLC, CD, SPIFE, UV/Vis, Enzymatic assays, LAL,OCTET, FACS, 96/384 well color/fluorescent readers/scanners. , Immunochemical methods: Quantitative sandwich and competitive ELISAs, Dot blotting, immune precipitation, obtaining MABs, epitope binning analysis, affinity EC50. , DNA manipulation: DNA-fragments gel purification, DNA fragment mobility shift assay, digestion, Ligation, Plasmid DNA purification, Transformation, Agarose and PAGE separation of DNA restrictase or PCR products, PCR directed Site mutagenesis, mRNA purific, Cell Culture: Maintaining of mammalian-, insect- and fish- cells in adherent or suspended form. Transient transfection with Ca-Ph, Lpf, Fugen, PEI; infection with baculoviruses and Phages. Creating transfected cell lines. Fusion. Obtaining Hybridomas. , Cell assays: viability assay, fluorescent/visual microscopy, Cyto-staining, FACS, Cell FIA, Cell RIA., Protein conjugation of IgG, FABs, target proteins with Biotin, AP, HRP, fluorescent dyes, I-125, Protein-protein interaction: OCTET, Shift assays: HPLC, native PAGE; ELISA. ELISPOT, HTP instruments: OKTET, EPMotion5075, NEMESIS 7200 immune-Histo-Stain Robot, 20x96 well plate washer, 1-10ml Manufacturing Dispenser. , Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, PRIZM5, ImageQuant, EPBlue.

These are some of the questions we asked our climbers about their experiences with Sea Lane Biotechnologies: