Ucsf/Cvri Work Values

Daily Duties at Ucsf/Cvri:

Separation White Blood Cells, Purification DNA out of it, carrying out PCR based SNP analyses, explaining SPIFE gel (Immune-fixating) and adapting it to MassSpec. Operating EPMotion5075 robot in 384 well format. Wrote SOPs for operation robot.

What they like about Ucsf/Cvri:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Protein purification from mammal cells, tissue, E Coli, , Protein analyses, Protein Conjugation, Developing Immune assays, MAB/FAB Small MW molecular Screening, Phage display screening, Cell culture, transfection, protein producing, San Diego Relocation

Skills

Protein Purification methods: Salt precipitation/solubility,UltraFiltr., gradient centrifugation, column chromatography at normal and higher pressure: SEC, IEX, HP, RP,Hep., HA, IMEC, protA/G, Lectin, Affinity, AKTA-(Express, FPLC, Prime, Explorer); FPLC , Protein characterization methods: PAGE/Western, IEF, 2-D PAGE (Coomassie, Fluorescent, Silver, ECL), HPLC, CD, SPIFE, UV/Vis, Enzymatic assays, LAL,OCTET, FACS, 96/384 well color/fluorescent readers/scanners. , Immunochemical methods: Quantitative sandwich and competitive ELISAs, Dot blotting, immune precipitation, obtaining MABs, epitope binning analysis, affinity EC50. , DNA manipulation: DNA-fragments gel purification, DNA fragment mobility shift assay, digestion, Ligation, Plasmid DNA purification, Transformation, Agarose and PAGE separation of DNA restrictase or PCR products, PCR directed Site mutagenesis, mRNA purific, Cell Culture: Maintaining of mammalian-, insect- and fish- cells in adherent or suspended form. Transient transfection with Ca-Ph, Lpf, Fugen, PEI; infection with baculoviruses and Phages. Creating transfected cell lines. Fusion. Obtaining Hybridomas. , Cell assays: viability assay, fluorescent/visual microscopy, Cyto-staining, FACS, Cell FIA, Cell RIA., Protein conjugation of IgG, FABs, target proteins with Biotin, AP, HRP, fluorescent dyes, I-125, Protein-protein interaction: OCTET, Shift assays: HPLC, native PAGE; ELISA. ELISPOT, HTP instruments: OKTET, EPMotion5075, NEMESIS 7200 immune-Histo-Stain Robot, 20x96 well plate washer, 1-10ml Manufacturing Dispenser. , Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, PRIZM5, ImageQuant, EPBlue.

These are some of the questions we asked our climbers about their experiences with Ucsf/Cvri: