University Of Nebraska Lincoln Work Values

Daily Duties at University Of Nebraska Lincoln:

What they like about University Of Nebraska Lincoln:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

Scientist, Biochemist, Biochemistry, Enzymology, Enzymologist, Protein, Protein Engineering, Cell Biology, Antibody, Drug development, DNA

Skills

 RNA/DNA/protein sequence analysis using bioinformatics tools,  DNA/RNA isolation, electrophoresis, reverse transcription,  PCR & real-time qPCR,  Constructs design, cloning and site-directed mutagenesis,  Protein modeling, proteomics and mass-spectrometry data analysis,  Protein expression in bacterial, insect & mammalian cells,  Purification of native and denatured proteins, refolding,  All types of chromatography (ion exchange, size-exclusion (SEC), hydrophobic interaction (HIC) and affinity chromatography), and HPLC ,  Protein modification: cross-linking, labeling, conjugation,  Protein characterization: IP, SDS PAGE, native blue PAGE, IEF, ITC, DSC, western blotting, UV/Vis and fluorescence spectroscopy, mechanistic enzymology, enzyme assay development, ELISA,  Maintenance of mammalian (HEK, COS, HeLa, podocytes) and insect (Sf21) cells and cell-based assay development,  Engineering, isolation and titer determination of viruses,  Transformation, transfection, viral transduction,  Generation and maintaining of stable cell lines, RNAi,  Immunocytochemistry, immunohistochemistry, Microscopy, FLIM-FRET

These are some of the questions we asked our climbers about their experiences with University Of Nebraska Lincoln: