Brigham And Women’s Hospital & Harvard Medical School Work Values

Daily Duties at Brigham And Women’s Hospital & Harvard Medical School:

What they like about Brigham And Women’s Hospital & Harvard Medical School:

The social vibrance of a hiring firm is very important to you. Your ability to make and maintain friendships there is a critical part of your decision. You would likely be dissatisfied with a workplace that is quiet, cold, or otherwise not particularly social. When you investigate a new hiring company, ask recruiters, managers, and potential co-workers about the social life and opportunities there. This is especially important when you are relocating; moving dramatically alters your social sphere both inside and outside the workplace.

Tags

Scientist, Biochemist, Biochemistry, Enzymology, Enzymologist, Protein, Protein Engineering, Cell Biology, Antibody, Drug development, DNA

Skills

 RNA/DNA/protein sequence analysis using bioinformatics tools,  DNA/RNA isolation, electrophoresis, reverse transcription,  PCR & real-time qPCR,  Constructs design, cloning and site-directed mutagenesis,  Protein modeling, proteomics and mass-spectrometry data analysis,  Protein expression in bacterial, insect & mammalian cells,  Purification of native and denatured proteins, refolding,  All types of chromatography (ion exchange, size-exclusion (SEC), hydrophobic interaction (HIC) and affinity chromatography), and HPLC ,  Protein modification: cross-linking, labeling, conjugation,  Protein characterization: IP, SDS PAGE, native blue PAGE, IEF, ITC, DSC, western blotting, UV/Vis and fluorescence spectroscopy, mechanistic enzymology, enzyme assay development, ELISA,  Maintenance of mammalian (HEK, COS, HeLa, podocytes) and insect (Sf21) cells and cell-based assay development,  Engineering, isolation and titer determination of viruses,  Transformation, transfection, viral transduction,  Generation and maintaining of stable cell lines, RNAi,  Immunocytochemistry, immunohistochemistry, Microscopy, FLIM-FRET

These are some of the questions we asked our climbers about their experiences with Brigham And Women’s Hospital & Harvard Medical School: