Brigham And Women’s Hospital & Harvard Medical School Work Values

Daily Duties at Brigham And Women’s Hospital & Harvard Medical School:

What they like about Brigham And Women’s Hospital & Harvard Medical School:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Scientist, Biochemist, Biochemistry, Enzymology, Enzymologist, Protein, Protein Engineering, Cell Biology, Antibody, Drug development, DNA

Skills

 RNA/DNA/protein sequence analysis using bioinformatics tools,  DNA/RNA isolation, electrophoresis, reverse transcription,  PCR & real-time qPCR,  Constructs design, cloning and site-directed mutagenesis,  Protein modeling, proteomics and mass-spectrometry data analysis,  Protein expression in bacterial, insect & mammalian cells,  Purification of native and denatured proteins, refolding,  All types of chromatography (ion exchange, size-exclusion (SEC), hydrophobic interaction (HIC) and affinity chromatography), and HPLC ,  Protein modification: cross-linking, labeling, conjugation,  Protein characterization: IP, SDS PAGE, native blue PAGE, IEF, ITC, DSC, western blotting, UV/Vis and fluorescence spectroscopy, mechanistic enzymology, enzyme assay development, ELISA,  Maintenance of mammalian (HEK, COS, HeLa, podocytes) and insect (Sf21) cells and cell-based assay development,  Engineering, isolation and titer determination of viruses,  Transformation, transfection, viral transduction,  Generation and maintaining of stable cell lines, RNAi,  Immunocytochemistry, immunohistochemistry, Microscopy, FLIM-FRET

These are some of the questions we asked our climbers about their experiences with Brigham And Women’s Hospital & Harvard Medical School: