Massachusetts General Hospital & Harvard Medical School Work Values

Daily Duties at Massachusetts General Hospital & Harvard Medical School:

What they like about Massachusetts General Hospital & Harvard Medical School:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Scientist, Biochemist, Biochemistry, Enzymology, Enzymologist, Protein, Protein Engineering, Cell Biology, Antibody, Drug development, DNA

Skills

 RNA/DNA/protein sequence analysis using bioinformatics tools,  DNA/RNA isolation, electrophoresis, reverse transcription,  PCR & real-time qPCR,  Constructs design, cloning and site-directed mutagenesis,  Protein modeling, proteomics and mass-spectrometry data analysis,  Protein expression in bacterial, insect & mammalian cells,  Purification of native and denatured proteins, refolding,  All types of chromatography (ion exchange, size-exclusion (SEC), hydrophobic interaction (HIC) and affinity chromatography), and HPLC ,  Protein modification: cross-linking, labeling, conjugation,  Protein characterization: IP, SDS PAGE, native blue PAGE, IEF, ITC, DSC, western blotting, UV/Vis and fluorescence spectroscopy, mechanistic enzymology, enzyme assay development, ELISA,  Maintenance of mammalian (HEK, COS, HeLa, podocytes) and insect (Sf21) cells and cell-based assay development,  Engineering, isolation and titer determination of viruses,  Transformation, transfection, viral transduction,  Generation and maintaining of stable cell lines, RNAi,  Immunocytochemistry, immunohistochemistry, Microscopy, FLIM-FRET

These are some of the questions we asked our climbers about their experiences with Massachusetts General Hospital & Harvard Medical School: