Indian Institute Of Science Work Values

Daily Duties at Indian Institute Of Science:

*Studied the expression and functional characteristics of lysophosphatidylcholine acyltransferase, a part of multi-protein complex in microsomal membranes of immature peanut seeds *Protein extraction and purification from peanut seeds using biochemical methods *Identified the complex protein using 2D electrophoresis and MALDI-TOF analysis *Protein enzyme activity was confirmed by colorimetric and radiometric enzyme assays

What they like about Indian Institute Of Science:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Molecular biology , Cell Culture , Vaccination , Biochemistry , Biological research

Skills

Molecular Biology: Gene Cloning, Plasmid isolation, PCR, Real Time PCR, Reverse transcriptase PCR, DNA and RNA isolation, Prokaryotic & Eukaryotic expression and purification, transfection by Lipofectamine., Cell biology: In-vivo & in-vitro parasite culturing, maintaining primary cells (CHO, HeLa & HFF), Transfection & protein expression in mammalian cells, Fluorescent tagged protein., Immunology: Spleencyte isolation, ELISA, Antibody Isotype, Proliferation assay, Cytokine assay, Antibody titter, Immunoblotting, Screening with human serum. , Protein Chemistry: Protein detection and assay methods, Desalting and concentration of protein samples, Thin layer chromatography, Size exclusion chromatography, Basic principles of HPLC, Reverse-phase chromatography, Ion exchange and Affinity chromatogr, Animal Handling Experience in working with small animal models (mice, rats), Injections (intravenous, intraperitoneally, intramuscular and subcutaneous) Retro-orbital bleeding.

These are some of the questions we asked our climbers about their experiences with Indian Institute Of Science: