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Messages Pharmaceuticals, Inc Work Values
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Daily Duties at Messages Pharmaceuticals, Inc:
• Northern Blotting: Processed RNA from cells; processed gels and transferred the blots; radiolabelled w/ 32P-dCTP, scanned the blot using a Phosphoimager; analyzed the data; calculated percentage of mRNA expression. Processed polysome gradients via Northern and determined the molecular weight distribution of various RNA of interest. • PCR: Cloned out specific DNA fragments using designed sense and anti-sense primers. • Primer Design: Designed primers with correct melting temperature, percent of GC content, and an optimal length to achieve the optimum yield from parental DNA template. • Gel Shift Assay: Performed validation studies involving the binding of radiolabelled RNA to protein extracts to determine the specificity of certain RNA-protein interactions. • Sequencing: Sequenced specific DNA regions to check for any gene mutations before any further experimentation was performed by the target identification/development group. • Tissue Culture: Performed cell passaging. Treated cells with various compounds of interest and processed these cells for RNA studies. • Cloning: Molecular biology techniques including subcloning, ligation, and transformation. Knowledge in cloning vectors such as TOPO, TOP10F1’, pGL3, pRc/RSV, pBluescript.
What they like about Messages Pharmaceuticals, Inc:
Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.
Tags
LSR Fortessa, Animal models, in vitro assay, in vivo assays, ELISA, Flow cytometry, BSL-2 infectious lab, cell-based assays, Patient sample procurement
Information about Messages Pharmaceuticals, Inc
Company Rank: Not Available
Average length of employment : 4 years
Average salary of employees: $77,000
These are some of the questions we asked our climbers about their experiences with Messages Pharmaceuticals, Inc:
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| Were your performance expectations clearly communicated? | 0.0 |
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| Were you recognized for meeting or exceeding expectations? | 0.0 |
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| Did you feel like your personal contribution was important? | 0.0 |
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| Was your career path clearly outlined and discussed? | 0.0 |
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| I would recommend this as a place of employment. | 0.0 | |
| I believe in the purpose of this organization. | 0.0 | |
| I would work for this organization again. | 0.0 | |
| I feel employees are fairly compensated. | 0.0 |
Climbers who worked at Messages Pharmaceuticals, Inc had these interests:
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| Linkedin https://www.linkedin.com |
a business-oriented social networking service |
