Messages Pharmaceuticals, Inc Work Values

Daily Duties at Messages Pharmaceuticals, Inc:

• Northern Blotting: Processed RNA from cells; processed gels and transferred the blots; radiolabelled w/ 32P-dCTP, scanned the blot using a Phosphoimager; analyzed the data; calculated percentage of mRNA expression. Processed polysome gradients via Northern and determined the molecular weight distribution of various RNA of interest. • PCR: Cloned out specific DNA fragments using designed sense and anti-sense primers. • Primer Design: Designed primers with correct melting temperature, percent of GC content, and an optimal length to achieve the optimum yield from parental DNA template. • Gel Shift Assay: Performed validation studies involving the binding of radiolabelled RNA to protein extracts to determine the specificity of certain RNA-protein interactions. • Sequencing: Sequenced specific DNA regions to check for any gene mutations before any further experimentation was performed by the target identification/development group. • Tissue Culture: Performed cell passaging. Treated cells with various compounds of interest and processed these cells for RNA studies. • Cloning: Molecular biology techniques including subcloning, ligation, and transformation. Knowledge in cloning vectors such as TOPO, TOP10F1’, pGL3, pRc/RSV, pBluescript.

What they like about Messages Pharmaceuticals, Inc:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

LSR Fortessa, Animal models, in vitro assay, in vivo assays, ELISA, Flow cytometry, BSL-2 infectious lab, cell-based assays, Patient sample procurement

Skills

Flow Cytometry , Cell-based ELISA, in vivo mouse models, in vitro assays

These are some of the questions we asked our climbers about their experiences with Messages Pharmaceuticals, Inc: