University Of Toledo, College Of Medicine And Life Sciences Work Values

Daily Duties at University Of Toledo, College Of Medicine And Life Sciences:

Compiled preliminary data for a National Heart, Lung, and Blood Institute (NHLBI) grant application with a projected budget of more than $1.5M annually. Managed the development of 26 congenic rat strains with a substitution genetic mapping strategy, monitoring a colony of over 500 rats, resulting in the genotyping of more than 3,000 rats in a year. Genetic mapping of the region on rat chromosome 1, 9, and 10 involved in the blood pressure regulation. Identification of the disease causative genetic factor/gene based on its location on the rat genome by linkage analysis and Quantitative Trait Locus (QTL) mapping by substitution mapping and/or gene expression and protein expression profiling using whole genome systems biology approaches. Performed cell biology and molecular biology experiments that resulted in high-impact publications.

What they like about University Of Toledo, College Of Medicine And Life Sciences:

The social vibrance of a hiring firm is very important to you. Your ability to make and maintain friendships there is a critical part of your decision. You would likely be dissatisfied with a workplace that is quiet, cold, or otherwise not particularly social. When you investigate a new hiring company, ask recruiters, managers, and potential co-workers about the social life and opportunities there. This is especially important when you are relocating; moving dramatically alters your social sphere both inside and outside the workplace.

Tags

Molecular biology, Next generation sequencing (NGS), Genomics, Genetics, Cardiovascular disease, Animal models, QTL mapping

Skills

As a lab manager, involved in the recruitment, familiar with SOPs/ GLPs, training and supervision of graduate students, postdoctoral fellows, and technicians., Worked closely with the Principal Investigators and collaborators to monitor the research grants progress., Successful at independently designing and executing scientific projects, writing IACUC protocols, method development, analyzing and interpreting data, and writing scientific reports, manuscripts and grant proposals. , Well versed in next generation DNA and RNA sequencing and large-scale data analysis, QTL mapping, Microarray and IPA analysis, Expert in molecular biology techniques including, RTPCR, qRTPCR, cloning, over-expression of recombinant proteins, protein purification, Western blot, ELISA, cDNA and NGS library construction, Excellent at basic microbiology and biochemistry techniques; Maintenance of various mammalian cell lines, siRNA design, lentiviral vector manipulation, generation of stable knockdown cell lines, Have advanced knowledge in Immunofluorescence, CHIP assay, Flow cytometry. Genome editing using ZFNs and TALENs, Radio- telemetry surgery (C10 and C40 probes) for blood pressure and ECG measurements., knowledgeable of Sequencher DNA Sequence Analysis Software, Galaxy, Intergrated Genome Browser, SeqMonk and Avadis NGS Softwares, IPA Analysis, GeneSpring, Extensive knowledge in public genomic data resources of the NCBI, UCSC and ENSEBL.

These are some of the questions we asked our climbers about their experiences with University Of Toledo, College Of Medicine And Life Sciences: