Louisiana State University Health Science Center Work Values

Daily Duties at Louisiana State University Health Science Center:

To conduct clinical trail to evaluate antiviral drug on human subjects. To develop an assay to determine viral DNA in human tear and saliva samples. To identify interaction of drug using in vitro and in vivo methods. To collaborate scientist for advancement of projects

What they like about Louisiana State University Health Science Center:

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Infectious disease, Oncology, Animal model, Immunology, Scientist, Host responses

Skills

➢ Cell based assays: Cytotoxicity assays, spectrophotometry, Drug inhibition assays, Apoptosis assays, Chip assays, Calorimetric biochemical assays. Luciferase reporter assays, Proliferation assays, Kinase assays. , ➢ Immunological techniques/Antibody characterization: Western blots, ELISA, Immunoprecipitation, Immunofluorescence, immunohistochemistry, ELIspot, Antibody purification. , ➢ Protein Expression analysis: Transfection, Transduction, Preparing stable cell lines, SDS-PAGE, Bacterial Transformation, Bradford & BCA analysis of proteins, Post-transcriptional analysis (dephosphorylation (phosphatase), phosphorylation (kinases), u, ➢ DNA/RNA manipulation techniques: Molecular Biology techniques, Molecular cloning, Site directed mutagenesis, Plasmid purification, restriction enzyme, dephosphorylation of DNA, ligation, Sequence analysis, RNAi, siRNA & shRNA mediated gene silencing. , ➢ Microscopy/Imaging skills: Immunofluorescence, Confocal microscopy, Fluorescence microscopy, Deconvolution microscopy, slit-lamp microscopy. , ➢ Cell Culture/ Tissue culture: Mammalian/Mouse/Rat/dog/Bacterial/Fungal cell culture, Epithelial/ Neural/Liver/Corneal/T-cell/Tumor cell lines, storage and maintenance, Establishment of genetically engineered stable cell lines, primary cell culture, ce, ➢ Drug Development: Small molecule drug screening, Drug resistance studies, CLSI approved drug screening assays, in-vivo drug screening, synergistic interaction of drugs (Fractional inhibitory (FICI) assay, combination index (CI) assay) , ➢ Animal Work: Maintenance of mouse colonies, infectious diseases mouse and rabbit models, in-vivo models, in-vivo screening of drugs, mouse anatomy, tissue harvesting and storage, Immunization, intramuscular, intravenous, intra-peritoneal injections, o, ➢ Microbiology techniques: Bacterial/ fungal/ viral culture and storage, antibacterial, antifungal and antiviral screening, plaque assays, Growth curves, time kill assays, neutralization assays., ➢ Laboratory management: Training of new graduate students, trainee, postdoctoral fellows, critical evaluation of data, problem solving skill, Write manuscripts and grant proposals, Presentation of data in institutional/international meetings, Manage la, ➢ Computer skills: Microsoft office, NIS elements, Image J, calcusyn, clustal W, Bioinformatic and statistical tools.

These are some of the questions we asked our climbers about their experiences with Louisiana State University Health Science Center: