University Of Arizona Work Values

Daily Duties at University Of Arizona:

Study the biosynthesis of antibiotics enterocin produced by marine bacterium Streptomyces maritimus, as well as supporting other projects.

What they like about University Of Arizona:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Process Development Professional, Fermentation and Primary Recovery, Cell Banking, Protein Engineering, Genetic Engineering, Metabolic Engineering

Skills

Microbe isolation, culturing and characterization, Developing and characterizing fermentation and primary recovery process to achieve effecient production of therapeutic proteins, plasmids, and antibiotics by using flasks, DasGip, and fermentors., Developing microbe genetic systems for solving various issues especially related to strains with strong genetic obstacles. Knocking out of genes, metabolic engineering, and mutasynthesis., Construction of various plasmids for constructing gene library, cloning, heterologous expression, and protein expression in microbe and mammalian cells. Protein expression, purification, and characterization in microbe and mammalian cells., Solid skills in cloning and expression of genes (known and unknown), DNA and RNA manipulation(isolation, PCR and QRT-PCR, cDNA synthesis, southern hybridization, site-directed mutagenesis), DNA sequence analysis, and Western blot., Precisely quantifying fermentation sample protein titers (SDS-PAGE) and plasmid titers. Strong skills in extraction, isolation (column chromatography and HPLC), and characterization (HPLC, TLC, LC-MS, NMR) of chemicals from bacterial fermentation broth., Successfully writing SOPs and batch records of fermentation process and primary recovery., Strong skills in Microsoft Word, PowerPoint, and Excel, Statistics analysis program, ChemDraw, BioEdit, GCG, Sequencer, Vector IT15 (for DNA and Protein Analysis), and SDS (for QRT-PCR analysis).

These are some of the questions we asked our climbers about their experiences with University Of Arizona: