Human Biomolecular Research Institute Work Values

Daily Duties at Human Biomolecular Research Institute:

Protein engineering and characterization; Analyze gene transcription of clinical blood samples

What they like about Human Biomolecular Research Institute:

A hiring company that offers alternative approaches to pay, performance bonuses, and/or equity in the firm is very attractive to you. You're generally less interested in working for an organization offering only traditional, salary-based compensation, one with limited opportunity to acquire equity in the firm, or one that does not actively promote skill development. In addition, you are more likely to choose a company that offers a variety of internal career options, with defined career paths. You view the learning of new skills and development of your expertise as key to your career advancement. These aspects of an organization may become even more important to you as you progress in your field, and are especially critical if you change career or occupation.

Tags

Process Development Professional, Fermentation and Primary Recovery, Cell Banking, Protein Engineering, Genetic Engineering, Metabolic Engineering

Skills

Microbe isolation, culturing and characterization, Developing and characterizing fermentation and primary recovery process to achieve effecient production of therapeutic proteins, plasmids, and antibiotics by using flasks, DasGip, and fermentors., Developing microbe genetic systems for solving various issues especially related to strains with strong genetic obstacles. Knocking out of genes, metabolic engineering, and mutasynthesis., Construction of various plasmids for constructing gene library, cloning, heterologous expression, and protein expression in microbe and mammalian cells. Protein expression, purification, and characterization in microbe and mammalian cells., Solid skills in cloning and expression of genes (known and unknown), DNA and RNA manipulation(isolation, PCR and QRT-PCR, cDNA synthesis, southern hybridization, site-directed mutagenesis), DNA sequence analysis, and Western blot., Precisely quantifying fermentation sample protein titers (SDS-PAGE) and plasmid titers. Strong skills in extraction, isolation (column chromatography and HPLC), and characterization (HPLC, TLC, LC-MS, NMR) of chemicals from bacterial fermentation broth., Successfully writing SOPs and batch records of fermentation process and primary recovery., Strong skills in Microsoft Word, PowerPoint, and Excel, Statistics analysis program, ChemDraw, BioEdit, GCG, Sequencer, Vector IT15 (for DNA and Protein Analysis), and SDS (for QRT-PCR analysis).

These are some of the questions we asked our climbers about their experiences with Human Biomolecular Research Institute: