Tufts University School Of Medicine Work Values

Daily Duties at Tufts University School Of Medicine:

Understand the molecular regulation of the DNA tumor virus oncogenes in increasing sensitivity to DNA damaging agents, chemo therapeutics and radiation therapy in mammalian cells. Targeted Mutational Analysis of the Oncogene to identify and characterize its functions and striking phenotypic effects in the mammalian cells.

What they like about Tufts University School Of Medicine:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS

Skills

RNAi RNA knockdown (Transient siRNA or Stable shRNA knockdowns Lentivirus or Retrovirus Mediated), Gene Overexpression, PCR RTPCR , Recombinant DNA Cloning Subcloning to Expression Vectors, Plasmid Design & Construction Restriction Digestion Ligation Sequencing (DNA & Protein), Construction of Stable Tet-Inducer Cell Lines, Preparation and Infection : Lentivirus, Retrovirus, Polyomavirus A2 or NG59RA, Migration, Invasion, Proliferation Assays, Phenotyping Mammalian Cell Lines, Primary Cell Culture, Transfection (Transient Fwd/Rev), Stable (Single Cell Cloning/ Viral Transduction), ELISA, Multicolor Flow Cytometry or FACS, Image Stream Analysis, Immunocytochemistry, Immunoflourescence, Focus Formation (Transformation) Assays, DNA Damage Detection (Comet Assays) TUNEL Staining & DNA Laddering For Apoptosis Detection, Co-Immunoprecipitation, Detection of Protein Modifications, Western Blots, Southern, Northern Blots, Microarray Analysis, Tandem Affinity Purification, Mass Spectrometry, Protein Kinase Activity Assay, Pulse Chase Analysis, Radio-labeling, Mitochondrial & Nuclear Extraction, Isolation of low molecular weight DNA or genomic DNA for quantification of DNA replication, Recombinant Protein Expression, Protein Purification in E.coli, Protein Quantification, Analyzing specific and non-specific DNA binding ability of proteins, DNA Replication Assays, Transient Luciferase Reporter Assays (Firefly & Renilla), Confocal & Flourescence Microscopy, Biochemical Characterization, Effect of Inhibitor and Activator Studies, Image Quant/J Adobe Photoshop Mass Spectrometry HPLC Alpha Imager/ View & Cell Quest BLAST/FASTA & CLUSTAW Vector NTI suite & Gene Spring MASCOT CASP/PANTHER WIN-LIST 3D/MODFIT LT Swiss PDB Viewer & JMOL MS Word/Excel/PowerPoint

These are some of the questions we asked our climbers about their experiences with Tufts University School Of Medicine: