Dana Farber Cancer Institute (Harvard Medical School) Work Values

Daily Duties at Dana Farber Cancer Institute (Harvard Medical School):

# Jul 2009 - Present, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA *Project 1: NSCLC radiosensitization by activation of apoptosis via DUBs inhibition • To identify novel radiosensitizers, we performed a whole genome pooled shRNA screen in NSCLC cell lines with fractionated ionizing radiation (IR). DUBs, a deubiquitinase, was a differential top hit. DUB knockdown via RNAi and inhibition via small molecule inhibitor shows synergistic cytotoxicity with IR in NSCLC and trigger apoptosis, we studied the pharmacokinetics (PK) and pharmacodynamics (PD) of DUB small molecule inhibitor on NSCLC for the potential use in clinical settings. • Research article describing this research work is under preparation *Project 2: Whole Genome RNAi Screen Identifies Proteasome Inhibition as a Strategy for NSCLC Radiosensitization • Novel radiosensitizers may improve the therapeutic index of RT for lung cancer patients. We performed whole genome pooled RNAi screens using the Hannon-Elledge library, in two NSCLC cell lines A549 and NCI-H460 • Proteasome inhibition emerged as a top drug target of an RNAi screen designed to identify targets with sustained activity over multiple cell generations. Its synergistic impact on NSCLC viability when coupled with fractionated RT should be further explored in preclinical studies and early clinical trials • Research article describing this research work is under preparation

What they like about Dana Farber Cancer Institute (Harvard Medical School):

You highly value a work environment built on a formal structure, rules, and regulations. You do not enjoy the unpredictability of shifting priorities and deadlines that upset your routine. You require and enjoy direction, input and accountability as part of your work environment. You have a strong need to participate in making key decisions and feel left out if your superiors or co-workers do not seek your input when making decisions. You thrive on providing good customer service to both internal or external customers, and doing so makes you feel good.

Tags

Drug Discovery, Target Validation, Molecular Biology, Cancer Research, invitro biology, invivo biology, translational research, functional genomics, Biomedical Research, Pharmacokinetics, Oncology, Biomarkers

Skills

Requirements Analysis & Specification, mammalian cell culture, colorimetric and fluorimetric assays, spectrophotometry DNA cloning, PCR, DNA ligation and transformation, enzyme activity assay and Western blot analysis, DNA Repair Assay, Sequencing PAGE Gels, miRNA HTS screen, RNAi HTS screen, miRNA, RNA, Protein and DNA isolation and analysis, QPCR, QRT PCR, MS PCR, Taqman PCR, ex vivo(Human and mouse tumor xenograft isolation and analysis), invivo study., expression constructs for mammalian, baculo-virus expression system, transfection and transformation to overexpress recombinant proteins, in vitro cell based assays, immunoassays, Small Molecule inhibitors IND experiments (Pharmaco Kinetics Study), Cell S, Experience in scientific writings (including grants, animal protocols) and maintaining good laboratory practices (GLPs), research collaboration and enthusiastic team player

These are some of the questions we asked our climbers about their experiences with Dana Farber Cancer Institute (Harvard Medical School):