Wayne State University Work Values

Daily Duties at Wayne State University:

# Aug 2004 – June 2009, Wayne State University & Karmanos Cancer Institute, Detroit, MI * PhD Thesis (Advisor: Dr. Ahmad R Heydari): Role of Uracil DNA glycosylase in BER pathway under folatedeficiency • The exact mechanism by which folate deficiency induces carcinogenesis is yet unclear. Folate deficiency increases the uracil pool which is repaired by DNA glycosylase initiated base excision repair (BER). • Thus, we identified whether DNA Glyocsylase (the first enzyme of BER pathway) silencing displays a protective phenotype under folate deficiency. So to do a proof of concept (POC) study we knocked down UDG and created a haploinsufficiency, then studied its affect on overall BER pathway and DNA damage response in tumor cells. • Research article describing this research work is submitted * Project 1 (2005-2007): Impact of Folate deficiency on epigenetic alterations of DNA and its role in Carcinogenesis • Folate deficiency alters the methylation pool with in the cell under oxidative stress (2NP), we next analyzed whether it effects promoter methylation of various BER genes, such as DNA glycolsylases, promoter have CpG islands. Additionally, we also established this could alter the transcription or enzyme activity of DNA glycosylases • Research article describing this research work is submitted

What they like about Wayne State University:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Drug Discovery, Target Validation, Molecular Biology, Cancer Research, invitro biology, invivo biology, translational research, functional genomics, Biomedical Research, Pharmacokinetics, Oncology, Biomarkers

Skills

Requirements Analysis & Specification, mammalian cell culture, colorimetric and fluorimetric assays, spectrophotometry DNA cloning, PCR, DNA ligation and transformation, enzyme activity assay and Western blot analysis, DNA Repair Assay, Sequencing PAGE Gels, miRNA HTS screen, RNAi HTS screen, miRNA, RNA, Protein and DNA isolation and analysis, QPCR, QRT PCR, MS PCR, Taqman PCR, ex vivo(Human and mouse tumor xenograft isolation and analysis), invivo study., expression constructs for mammalian, baculo-virus expression system, transfection and transformation to overexpress recombinant proteins, in vitro cell based assays, immunoassays, Small Molecule inhibitors IND experiments (Pharmaco Kinetics Study), Cell S, Experience in scientific writings (including grants, animal protocols) and maintaining good laboratory practices (GLPs), research collaboration and enthusiastic team player

These are some of the questions we asked our climbers about their experiences with Wayne State University: