Dana Farber Cancer Institute (Harvard Medical School) Work Values

Daily Duties at Dana Farber Cancer Institute (Harvard Medical School):

*Project 1: miRNA targeted post-transcriptional and post-translational regulation of MGMT in glioblastoma • To better understand mechanism of action (MOA) of MGMT and its post transcriptional regulations we did a Human genome wide miRNA screen in glioblastoma cell lines. miRNAs could serve as a more potent non-invasive biomarker. • Our functional analysis and preclinical data suggest that miRNAs could serve as a better predictive biomarker for TMZ response and that its role is mediated, in part, by post-transcriptional regulation of MGMT. • Research Article for miR-181d and its post transcriptional regulation of MGMT (published). Research article describing synergistic effect of miRNAs and MGMT interactions is in press *Project 2: Post-transcriptional regulation of O6-methylguanine-DNA methyltransferase MGMT in glioblastomas • The DNA repair enzyme (MGMT) confers therapeutic resistance to DNA alkylating agents, including temzolomide. Our mRNA data and protein levels analysis in a array of Glioblastoma tumor specimens and cell lines suggest poor correlation with MGMT DNA methylation status. Interestingly, in-silico analysis predicted potential binding sites for several miRNA within the 3'UTR of MGMT emphasizing post transcriptional regulation of MGMT in glioblastoma. • Research article describing this research work is published

What they like about Dana Farber Cancer Institute (Harvard Medical School):

The social vibrance of a hiring firm is very important to you. Your ability to make and maintain friendships there is a critical part of your decision. You would likely be dissatisfied with a workplace that is quiet, cold, or otherwise not particularly social. When you investigate a new hiring company, ask recruiters, managers, and potential co-workers about the social life and opportunities there. This is especially important when you are relocating; moving dramatically alters your social sphere both inside and outside the workplace.

Tags

Drug Discovery, Target Validation, Molecular Biology, Cancer Research, invitro biology, invivo biology, translational research, functional genomics, Biomedical Research, Pharmacokinetics, Oncology, Biomarkers

Skills

Requirements Analysis & Specification, mammalian cell culture, colorimetric and fluorimetric assays, spectrophotometry DNA cloning, PCR, DNA ligation and transformation, enzyme activity assay and Western blot analysis, DNA Repair Assay, Sequencing PAGE Gels, miRNA HTS screen, RNAi HTS screen, miRNA, RNA, Protein and DNA isolation and analysis, QPCR, QRT PCR, MS PCR, Taqman PCR, ex vivo(Human and mouse tumor xenograft isolation and analysis), invivo study., expression constructs for mammalian, baculo-virus expression system, transfection and transformation to overexpress recombinant proteins, in vitro cell based assays, immunoassays, Small Molecule inhibitors IND experiments (Pharmaco Kinetics Study), Cell S, Experience in scientific writings (including grants, animal protocols) and maintaining good laboratory practices (GLPs), research collaboration and enthusiastic team player

These are some of the questions we asked our climbers about their experiences with Dana Farber Cancer Institute (Harvard Medical School):