Veterans Administration, Greater Los Angeles Work Values

Daily Duties at Veterans Administration, Greater Los Angeles:

My daily task is to train two technicians and two research fellows in the following techniques, interpret data and troubleshoot problems on a daily basis that arise while the experiments are being carried out. a) Producing genetic expression vectors to express antibodies and novel proteins by teaching them how to manipulate DNA fragments using the Software Gene Construction Kit, how to design primers, how to set up PCR, and how to clone PCR fragments into expression vectors. b) Express the antibodies and novel proteins in myeloma and 293 cells c) Grow transfectants in large cultures, and to purify antibodies and novel proteins from culture supernatants by affinity chromatography using AKTA purifier. d) Characterizing proteins by SDS-PAGE, Western Blotting and ELISA. e) Characterize the proteins in vitro using cell lines and in mice. f) To write up new protocols. g) To write manuscripts to publish in scientific journals. h) To write proposals to obtain funding for research.

What they like about Veterans Administration, Greater Los Angeles:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Antibody engineering, Protein engineering, Gene transfection technology, Training undergraduate students, Training graduate students, Training post-graduate scholars, Teaching

Skills

P&L responsibilities, Ecommerce Strategy and digital marketing channels optimization - SEO, SEM, email, affiliates - for high-profile accounts., Technology Solutions, Sales Support, Software Implementation, Requirements Analysis & Specification, Genetic engineering and gene transfection technology, Protein engineering, Proposal writing, Writing patents, Negotiating patents, Writing research manuscripts, Lab and personel managment, Teaching

These are some of the questions we asked our climbers about their experiences with Veterans Administration, Greater Los Angeles: