Sealane Biotechnologies. Work Values

Daily Duties at Sealane Biotechnologies. :

Screening of phage display produced bacterial clones, purification and analyses of FABs, developing new quantitative and qualitative immune-assays for different antigens,conjugating proteins with biotin, dyes, enzymes. Purification DNA.

What they like about Sealane Biotechnologies. :

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Protein purification and Analyses, Development immune Assays, obtaining and analyses monoclonal antibodies or FABs, Cell culture work

Skills

Software Implementation, Editing skills and developing methods in protein biochemistry, immune assay and cell assay, Computer skills: MS Word, Excel, Power Point, SofMaxPro, Unicorn, ImageQuant., Project planning from design through evaluation, with expertise in proper survey design and application of qualitative and quantitative techniques in FAB Phage display screening, Excellent problem-solving skills (systematically analyzing and deconstructing problems, building consensus for group solutions), Protein Purification methods: column chromatography at normal and higher pressure: Size exclusion Gel chromatography, ion exchange, hydrophobic, reverse phase, affinity- and ligand- chromatography, Protein characterization: PAGE/Western, IEF, 2-D electrophoresis. HPLC, studied Protein-protein interaction with ELISA, measured interaction affinity, Circular Dichroism, Surface Plasmon resonance assay (OKTET)., Biochemical and Enzymology methods: Measurement of proteins (A280,Bradford, BCA, Lowry, Coomassi binding assay), Activity measurement of AP, HRP, Alkaline Pyrophosphatases, Protein Kinase, Proteinase, Endotoxines, conjugation of proteins., Immunological methods: Quantitative sandwich and competitive ELISAs, Western blot, Dot blotting, immune precipitation, epitope binning analysis MAB/FAB, Cell Culture: Maintaining of mammalian-, insect- and fish- cell lines in adherent and suspended form. Transient transfection with Ca-Phosphate, Lipofectamin, Fugen,PEI, obtaining MABs, FACS.

These are some of the questions we asked our climbers about their experiences with Sealane Biotechnologies. :