University Of Pennsylvania Work Values

Daily Duties at University Of Pennsylvania:

• Design, construction and characterization of novel attenuated and recombinant Listerial vaccine vectors expressing HIV-1/SIV, or SHIV genes: such as IPTG inducible system, ssrA proteolysis tagging system (enhancing protein degradation), chromosomal integration system (different integration sites). The gene expression and translation were examined in details at the molecular levels (plasmid copy number, mRNA level, intracellular protein level). • Vaccine vector optimization, production and QC assay development for primate studies. • Studying mucosal immune responses by mucosal route delivery of Listerial vaccines (intranasal, oral, intravaginal and intrarectal immunization). • Culture, purification and characterization of recombinant adenovirus expressing HIV or SIV genes. • Studying the safety and immunogenicity of these recombinant strains in mice, especially examining the systemic and mucosal immune responses to HIV-1 or SIV of recombinant vaccines in heterologous immunization strategy (DNA or RNA vaccines, virus-like particles, dendritic cells, Listeria, adenovirus 5 or 26, poxvirus vaccines); Analysis of the polyfunctionality (CD107a, TNF-alpha, IL-2, IFN-gamma secretion ability) and phenotypes of antigen-specific CD8 T cells in effector, effector memory and central memory. • In vivo depletion of CD4 and CD8 T cells with monoclonal antibodies; Extensive study of in vivo cytolysis activity using CFSE labeling assay in many tissues (vagina, MLN, ILN, liver, Lung, spleen); Good at the adoptive transfer experiments. • Development of novel nucleotide-modified pseudouridine-containing mRNA-based HIV vaccines (mRNA, mRNA-loaded dendritic cells), and their formulation; Test the immune responses with mRNA as vaccines in mice in prime/boost strategy (Listeria /mRNA, Listeria/ DC, mRNA/adenovirus). • Attend the oncology seminars to keep updated knowledge in cancer cell biology, biomarkers identification and target therapies.

What they like about University Of Pennsylvania:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

Microbiologist, bacteriology, viologist, immunologist, molecular biologist, animal scientist

Skills

P&L responsibilities, Presentation, Ecommerce Strategy and digital marketing channels optimization - SEO, SEM, email, affiliates - for high-profile accounts., Technology Solutions, Sales Support, Software Implementation, Requirements Analysis & Specification, Leadership , Working independently and collaboratively, Familiar with the techniques used in molecular biology, immunology, microbiology (bacteriology and virology), and animal experiments.

These are some of the questions we asked our climbers about their experiences with University Of Pennsylvania: