Moffitt Cancer Center Work Values

Daily Duties at Moffitt Cancer Center:

•Studies on signal transduction and its effect on malignancy: Studying the signaling involved in transcriptional regulation of MUC4 in upregulating pancreatic cancer. •Protein–DNA interactions studies (chIP analysis), Gene Reporter assays (luciferase assay, CAT assay). •Studied the assigned tasks thoroughly before the actual experimentation and discussed doubts with the senior scientists. •Studied structure to function relationship including optimization of cell-based and bioactivity assays (viability, cytotoxicity, and apoptosis, etc.), enzymatic activity and inhibition characterization, immunochemistry, and mycoplasma testing.

What they like about Moffitt Cancer Center:

Working for a company with an emphasis on social values and helping society is a clear and important priority for you - a critical factor in who you choose to work for. In contrast to other factors, you place an organization's reputation for fairness and concern for the community above most other aspects of the company. As you search for a new job opportunity, it is usually possible to find out if the company is involved in the community and/or if it is addressing problems and issues in society. Pay special attention to non-profit organizations and those specifically involved in social action. Moreover, speaking with current and former employees should enlighten you to perceptions of the fairness of the company's leaders and the treatment of employees.

Tags

Research, Molecular Biology, Biochemistry, Cell Biologist, Drug Discovery, Cell Culture

Skills

Drug Discovery: FPLC, HPLC, protein purification techniques- gel filtrations and affinity chromatography, SDS-PAGE, Western blotting, RIA, ELISA, fluorescence microscopy, Immunohisto/cytochemistry, Fibrin and Gelatin zymography, EMSA, Microinjection and , Molecular biology:Transformation, restriction analysis, RT-PCR, cloning and Gene knock out experiments, Nuclease protection assay, RNAi techniques, adenoviral and retroviral mode of transfection, Single cell proliferation assay, Southern and Northern blot, Cell culture:Isolation of vascular smooth muscle cells from the aorta of mice, rat and rabbit, Isolation of monocytes and neutrophils from peripheral blood from human and mice, isolation of endothelial cells from human umbilical cord, Coculture system, Ra, Others: FACS analysis, Chromatin Immunoprecipitation (chIP) assay, qRT-PCR and other gene expression assays, CAT assay,Raising orthotopic breast tumors, pancreatic tumors and lung tumors in nude and SCID mice.

These are some of the questions we asked our climbers about their experiences with Moffitt Cancer Center: