University Of Illinois At Chicago Work Values

Daily Duties at University Of Illinois At Chicago:

• Worked on a doctoral thesis research, studying the membrane binding and activation mechanisms of Phospholipase A2 and 5-Lipoxygenase enzymes which required protein expression and purification using both bacterial and insect cell lines, protein refolding/engineering, enzyme activity assays, and DNA mutagenesis. Teaching and coordination of Chemistry, Biochemistry and general chemistry lab courses.

What they like about University Of Illinois At Chicago:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

proteomics, peptide, protein characterization, protein/antibody purification, bioassay, LCMS

Skills

• Skilled at performing LCMS (ion trap, nano-LC-LTQ and nano-LC-LTQ-FTICR), confocal, and multiphoton microscope imaging, coupled with expertise in operating surface plasmon resonance (SPR, BiaCore), spectrofluorimeter, and plate readers., • Exemplify proven success in conducting protein expression in insect, mammalian, and bacterial systems as well as purification of protein/antibody using gel filtration, ion exchange, hydrophobic, and affinity chromatography with AKTA FPLC system , • Strong ability in peptide/protein characterization using mass spectrometry and proteomics database search engines, both completed in fiercely competitive and fast-paced environment, • Proficient in preparing samples for proteomics using 1-D, 2-D gel electrophoresis, tryptic digestions, and western blotting, • Offer unyielding expertise in molecular biology (PCR, construct preparation and cloning), qPCR, siRNA and isolation, and purification of DNA and RNA, • Possess in-depth knowledge of high-throughput assay development, including ELISA and enzyme assays, cell-based real time activity assays, fluorescence ligand binding assays, mass spectrometric assays, and radio ligand binding assays, • Acquire thorough knowledge of mammalian cell culture and transfection, • Expertly dealt with estrogen, nitric oxide, eicosanoid, apoptotic, and chemoprevention signaling pathways, • Display technical skills in MassMatrix, Mascot, PyMol, Microsoft Office Suite, ChemDraw, and Sigma plot

These are some of the questions we asked our climbers about their experiences with University Of Illinois At Chicago: